ELISA Kit for Coagulation Factor VII (F7)
FVII; SPCA; proconvertin; Cothromboplastin; Serum Prothrombin Conversation Accelerator; Stable Factor; Eptacog alfa
- Product No.SEB874Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypePlasma
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.78-50ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.31ng/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 684
For more details, please contact local distributors! US$ 3078
For more details, please contact local distributors! US$ 5814
For more details, please contact local distributors! US$ 47880
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Coagulation Factor VII (F7).
No significant cross-reactivity or interference between Coagulation Factor VII (F7) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Coagulation Factor VII (F7) and the recovery rates were calculated by comparing the measured value to the expected amount of Coagulation Factor VII (F7) in samples.
|Matrix||Recovery range (%)||Average(%)|
|sodium citrate plasma(n=5)||80-93||80|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor VII (F7) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor VII (F7) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Coagulation Factor VII (F7) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|sodium citrate plasma(n=5)||87-94%||83-90%||92-105%||92-105%|
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Am J Physiol Heart Circ Physiol.||Monocytes regulate systemic coagulation and inflammation in abdominal sepsis Pubmed:25502108|
|Perfusion||Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testing PubMed: 26243277|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|RPB874Mu01||Recombinant Coagulation Factor VII (F7)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPB874Mu02||Recombinant Coagulation Factor VII (F7)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB874Mu02||Polyclonal Antibody to Coagulation Factor VII (F7)||WB; IHC; ICC; IP.|
|PAB874Mu01||Polyclonal Antibody to Coagulation Factor VII (F7)||WB; IHC; ICC; IP.|
|LAB874Mu71||Biotin-Linked Polyclonal Antibody to Coagulation Factor VII (F7)||WB; IHC; ICC.|
|HEB874Mu||High Sensitive ELISA Kit for Coagulation Factor VII (F7)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SEB874Mu||ELISA Kit for Coagulation Factor VII (F7)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSB874Mu01||ELISA Kit DIY Materials for Coagulation Factor VII (F7)||Main materials for "Do It (ELISA Kit) Yourself".|