ELISA Kit for Plasminogen (Plg)
PL; Plasmin; Activation peptide; Angiostatin
- Product No.SEB236Bo
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- Sample TypePlasma
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range6.25-400ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 2.61ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Plasminogen (Plg).
No significant cross-reactivity or interference between Plasminogen (Plg) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Plasminogen (Plg) and the recovery rates were calculated by comparing the measured value to the expected amount of Plasminogen (Plg) in samples.
|Matrix||Recovery range (%)||Average(%)|
|sodium citrate plasma(n=5)||79-98||82|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Plasminogen (Plg) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Plasminogen (Plg) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Plasminogen (Plg) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|sodium citrate plasma(n=5)||97-104%||84-98%||86-102%||78-95%|
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|International Journal of Molecular Sciences||The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC Mdpi: Source|
|J Anim Physiol Anim Nutr (Berl).||Improved milk production through PG-PL system by provision of in-house shelter management in lactating Murrah buffaloes during winter season. pubmed:28084661|
|Neurotherapeutics||iTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Management pubmed:29247448|
|Veterinarski Arhiv||The influence of a modified micro-environment on stress and milk production through the plasminogen-plasmin system in Murrah buffaloes during the hot-humid … 10.24099:vet.arhiv.170113|
|Catalog No.||Related products for research use of Bos taurus; Bovine (Cattle) Organism species||Applications (RESEARCH USE ONLY!)|
|SEB236Bo||ELISA Kit for Plasminogen (Plg)||Enzyme-linked immunosorbent assay for Antigen Detection.|