ELISA Kit for Beta Catenin (β-catenin)
CTNNb1; bCat; b-Cat; CTNN-B1; Catenin Beta 1; Cadherin-Associated Protein
- Product No.SEB021Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.6pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Beta Catenin (β-catenin).
No significant cross-reactivity or interference between Beta Catenin (β-catenin) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta Catenin (β-catenin) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta Catenin (β-catenin) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Clinica Chimica Acta||Versican and its associated molecules: Potential diagnostic markers for multiple myeloma Pubmed:25623955|
|Journal of Bone and Mineral Research||Adverse effects of osteocytic constitutive activation of ?-catenin on bone strength and bone growth Pubmed:25639729|
|Am J Transl Res||Role of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive rats PubMed: 26396666|
|Scientific Reports||Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA. pubmed:28533512|
|Life Sciences||Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways. pubmed:28754618|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPB021Hu01||Recombinant Beta Catenin (β-catenin)||Positive Control; Immunogen; SDS-PAGE; WB.|
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|CAB021Hu22||Anti-Beta Catenin (β-catenin) Monoclonal Antibody||Loading Control of WB.|
|SEB021Hu||ELISA Kit for Beta Catenin (β-catenin)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSB021Hu01||ELISA Kit DIY Materials for Beta Catenin (β-catenin)||Main materials for "Do It(ELISA Kit) Yourself"|