ELISA Kit for Insulin Receptor (INSR)

CD220; ISR; HHF5; IR

Specificity

This assay has high sensitivity and excellent specificity for detection of Insulin Receptor (INSR).
No significant cross-reactivity or interference between Insulin Receptor (INSR) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Receptor (INSR) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Receptor (INSR) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
IOSR Journal of Applied Chemistry Molecular and Biochemical Effect of Neem Extract On Experimental Diabetes Org:Source
Biol Pharm Bull Age-Dependent Onset of Insulin Resistance in Insulin-Resistant Mice PubMed: 26632184
Nanomedicine A newly developed silymarin nanoformulation as a potential antidiabetic agent in experimental diabetes pubmed:27623396
American Journal of Translational Research Subcutaneous liraglutide ameliorates methylglyoxal-induced Alzheimer-like tau pathology and cognitive impairment by modulating tau hyperphosphorylation and glycogen synthase kinase-3β. pubmed:28337257
Biomedicine & Pharmacotherapy Vitamin D3 intake as regulator of insulin degrading enzyme and insulin receptor phosphorylationin diabetic rats. pubmed:27930980
Eur Neuropsychopharmacol. Regulation of insulin receptor phosphorylation in the brains of prenatally stressed rats: New insight into the benefits of antidepressant drug treatment. pubmed:28063625
台湾农业研究 (台灣農業研究67 (4): 355-364) 香菇柄水萃物改善糖尿病降低阿茲海默氏症罹病風險初探
Scientific Reports Mechanism underlying starvation-dependent modulation of olfactory behavior in Drosophila larva Pubmed: 32080342
ADVANCED RESEARCHES Prenatal Stress Induced Spatial Memory Deficit in a Sex-Specific Manner in Mice: Possible Involvement of Hippocampal Insulin Resistance
Current Biology Internal State Affects Local Neuron Function in an Early Sensory Processing Center to Shape Olfactory Behavior in Drosophila Larvae
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