ELISA Kit for Angiotensinogen (AGT)
ANHU; SERPINA8; Angiotensin; Renin Substrate; Serpin Peptidase Inhibitor,Clade A,Member 8(Alpha-1 Antiproteinase,Antitrypsin)
- Product No.SEA797Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range3.12-200ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.31ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 630
For more details, please contact local distributors! US$ 2835
For more details, please contact local distributors! US$ 5355
For more details, please contact local distributors! US$ 44100
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Angiotensinogen (AGT).
No significant cross-reactivity or interference between Angiotensinogen (AGT) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Angiotensinogen (AGT) and the recovery rates were calculated by comparing the measured value to the expected amount of Angiotensinogen (AGT) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensinogen (AGT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensinogen (AGT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angiotensinogen (AGT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Transplant International||Elevated urinary angiotensinogen a marker of intrarenal renin angiotensin system in hypertensive renal transplant recipients: does it play a role in development of proteinuria in hypertensive renal transplant patients? Wiley: source|
|Amyloid||The relation between urinary angiotensinogen and proteinuria in renal AA amyloidosis patients PubMed: 22320202|
|Pediatric Mephrology||Urinary angiotensinogen as a marker of intrarenal angiotensin II activity in adolescents with primary hypertension PubMed: PMC3661928|
|Acta Paediatrica||Urinary angiotensinogen as a novel marker of obstructive nephropathy in children Pubmed: 23772991|
|Journal of the Renin-Angiotensin-Aldosterone System||Association between urinary angiotensinogen, hypertension and proteinuria in pregnant women with preeclampsia Sagepub: Source|
|Pediatric Nephrology||MP707 CHARACTERISTICS AND OUTCOMES OF CHILDREN WITH AUTOSOMAL RECESSIVE POLYCYSTIC KIDNEY DISEASE COHORT FROM THE ESPN/ERA-EDTA REGISTRY Oxfordjournals:Source|
|journal of human hypertension||Relationship between blood pressure var PubMed: 26223347|
|PLoS One||Higher Serum Angiotensinogen Is an Indicator of IgA Vasculitis with Nephritis Revealed by Comparative Proteomes Analysis PubMed: 26098644|
|MKA||Korelasi pengukuran antropometri dengan tekanan darah dan angiotensinogen plasma pada dewasa Index.Php: Art|
|Journal of Human Hypertension||Relationship between blood pressure variability and renal activity of the renin-angiotensin system. pubmed:26223347|
|Acta Pharmacol Sin.||Pharmacological evidence: a new therapeutic approach to the treatment of chronic heart failure through SUR2B/Kir6.1 channel in endothelial cells. pubmed:27890915|
|Journal of Chromatography A||Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis S0021967317311858|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA797Hu02||Recombinant Angiotensinogen (AGT)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA797Hu01||Recombinant Angiotensinogen (AGT)||Positive Control; Immunogen; SDS-PAGE; WB.|
|EPA797Hu61||Eukaryotic Angiotensinogen (AGT)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA797Hu01||Polyclonal Antibody to Angiotensinogen (AGT)||WB; IHC; ICC; IP.|
|PAA797Hu02||Polyclonal Antibody to Angiotensinogen (AGT)||WB; IHC; ICC; IP.|
|MAA797Hu22||Monoclonal Antibody to Angiotensinogen (AGT)||WB; IHC; ICC; IP.|
|SEA797Hu||ELISA Kit for Angiotensinogen (AGT)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA797Hu||CLIA Kit for Angiotensinogen (AGT)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA797Hu01||ELISA Kit DIY Materials for Angiotensinogen (AGT)||Main materials for "Do It (ELISA Kit) Yourself".|