ELISA Kit for Thioredoxin (Trx)
TXN; ADF; SASP; TRDX; TRX1; ATL-derived factor; Surface-associated sulphydryl protein
- Product No.SEA702Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range31.2-2,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 12.7pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 630
For more details, please contact local distributors! US$ 2835
For more details, please contact local distributors! US$ 5355
For more details, please contact local distributors! US$ 44100
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Thioredoxin (Trx).
No significant cross-reactivity or interference between Thioredoxin (Trx) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Thioredoxin (Trx) and the recovery rates were calculated by comparing the measured value to the expected amount of Thioredoxin (Trx) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thioredoxin (Trx) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thioredoxin (Trx) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Thioredoxin (Trx) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Human Reproduction||The roles of thioredoxin and thioredoxin-binding protein-2 in endometriosis OxfordJournals: source|
|Exp Ther Med.||Altered glutamate cysteine ligase activity in peripheral blood mononuclear cells from patients with systemic lupus erythematosus Pubmed:Pmc4061188|
|Reproduction||Alteration in the intrafollicular thiol–redox system in infertile women with endometriosis Pubmed:25376627|
|Free Radical Research||Thalassemia major patients using iron chelators showed a reduced plasma thioredoxin level and reduced thioredoxin reductase activity, despite elevated oxidative stress Pubmed:25564095|
|Antioxidants||Comparison of the Pulmonary Oxidative Stress Caused by Intratracheal Instillation and Inhalation of NiO Nanoparticles when Equivalent Amounts of NiO Are Retained in the Lung. Pubmed:26797643|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA702Hu01||Recombinant Thioredoxin (Trx)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA702Hu01||Polyclonal Antibody to Thioredoxin (Trx)||WB; IHC; ICC; IP.|
|FAA702Hu01||Anti-Thioredoxin (Trx) Polyclonal Antibody||Flow cytometry.|
|LAA702Hu81||FITC-Linked Polyclonal Antibody to Thioredoxin (Trx)||WB; IHC; ICC; IF.|
|LAA702Hu71||Biotin-Linked Polyclonal Antibody to Thioredoxin (Trx)||WB; IHC; ICC.|
|MAA702Hu22||Monoclonal Antibody to Thioredoxin (Trx)||WB; IHC; ICC; IP.|
|SEA702Hu||ELISA Kit for Thioredoxin (Trx)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA702Hu||CLIA Kit for Thioredoxin (Trx)||Chemiluminescent immunoassay for Antigen Detection.|