ELISA Kit for Vascular Cell Adhesion Molecule 1 (VCAM1)

CD106; INCAM-100; L1CAM

Specificity

This assay has high sensitivity and excellent specificity for detection of Vascular Cell Adhesion Molecule 1 (VCAM1).
No significant cross-reactivity or interference between Vascular Cell Adhesion Molecule 1 (VCAM1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Vascular Cell Adhesion Molecule 1 (VCAM1) and the recovery rates were calculated by comparing the measured value to the expected amount of Vascular Cell Adhesion Molecule 1 (VCAM1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-98 95
EDTA plasma(n=5) 98-105 102
heparin plasma(n=5) 78-92 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 (VCAM1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 (VCAM1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vascular Cell Adhesion Molecule 1 (VCAM1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 99-105% 80-102% 87-99% 84-98%
EDTA plasma(n=5) 84-101% 97-105% 89-97% 78-97%
heparin plasma(n=5) 98-105% 80-101% 84-95% 97-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Amran et al. Lipids in Health and Disease Changes in the vascular cell adhesion molecule-1, intercellular adhesion molecule-1 and c-reactive protein following administration of aqueous extract of piper sarmentosum on experimental. BioMed: 1476511X
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International Journal of Food Sciences and Nutrition Serum lipid profile and inflammatory markers in the aorta of cholesterol-fed rats supplemented with extra virgin olive oil, sunflower oils and oil-products PubMed: 26401576
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Pakistan journal of pharmaceutical sciences Changes in blood pressure, vascular reactivity and inflammatory biomarkers following consumption of heated corn oil. pubmed:29084680
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