ELISA Kit for Vascular Cell Adhesion Molecule 1 (VCAM1)
CD106; INCAM-100; L1CAM
- Product No.SEA547Si
- Organism SpeciesRhesus monkey (Simian) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range78-5,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 35pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Vascular Cell Adhesion Molecule 1 (VCAM1).
No significant cross-reactivity or interference between Vascular Cell Adhesion Molecule 1 (VCAM1) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Vascular Cell Adhesion Molecule 1 (VCAM1) and the recovery rates were calculated by comparing the measured value to the expected amount of Vascular Cell Adhesion Molecule 1 (VCAM1) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 (VCAM1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 (VCAM1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vascular Cell Adhesion Molecule 1 (VCAM1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Amran et al. Lipids in Health and Disease||Changes in the vascular cell adhesion molecule-1, intercellular adhesion molecule-1 and c-reactive protein following administration of aqueous extract of piper sarmentosum on experimental. BioMed: 1476511X|
|Vascular Pharmacology||Long-term ethanol consumption initiates atherosclerosis in rat aorta through inflammatory stress and endothelial dysfunction ScienceDirect: S1537189112000705|
|Canadian Journal of Physiology and Pharmacology||Resveratrol abrogates adhesion molecules and protects against TNBS-induced ulcerative colitis in rats. PubMed: 22029500|
|British Journal of Medical and Health Sciences||EFFECTS OF ADRIAMYCIN, CISPLATIN, AND 5-FLUOROURACIL ON THE TESTES OF ALBINO RATS Researchgate: Source|
|Endocrine Metabolic & Immune Disordors Drug Targets.||Active immunization against tumor necrosis factor-alpha decreases proinflammatory cytokines, oxidative stress mediators and adhesion molecules risk factors in streptozotocin-induced diabetic rats. Pubmed: 23859636|
|Atherosclerosis||Increased monocyte adhesion by endothelial expression of VCAM-1 missense variation in vitro Pubmed: 24075742|
|Atherosclerosis||Activation of receptor for advanced glycation end products contributes to aortic remodeling and endothelial dysfunction in sinoaortic denervated rats. Pubmed: 23880178|
|BioMed research international||Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations Pubmed: 24199192|
|Arch Dis Child||PO-0699 Estimation Of Adhesion Molecules Intrauterine Growth Retardation (iugr) Newborns Bmj:Source|
|Arch Dis Child?||PO-0701 Causes Of Fetal Growth Restriction And Early Neonatal Outcome Bmj:Source|
|Arch Dis Child||PO-0698 Neonatal Resuscitation Practices In The Delivery Room In India: An Audit Using Videography Bmj:Source|
|Naunyn-Schmiedeberg's Archives of Pharmacology||Insights into the antiatherogenic molecular mechanisms of andrographolide against?Porphyromonas gingivalis-induced atherosclerosis in rabbits Pubmed:25172523|
|Nutr Res.||Curcumin attenuates adhesion molecules and matrix metalloproteinase expression in hypercholesterolemic rabbits Pubmed:25282128|
|Alexandria Journal of Medicine||Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in Sprague Dawley rats Sciencedirect:S2090506814000268|
|Canadian Journal of Physiology and Pharmacology||Effect of magnesium sulfate and thyroxine on inflammatory markers in a rat model of hypothyroidism Doi: Abs|
|Hum Exp Toxicol||Effect of agmatine on experimental vascular endothelial dysfunction PubMed: 26424770|
|International Journal of Food Sciences and Nutrition||Serum lipid profile and inflammatory markers in the aorta of cholesterol-fed rats supplemented with extra virgin olive oil, sunflower oils and oil-products PubMed: 26401576|
|J Trace Elem Med Biol||Comparative angioprotective effects of magnesium compounds PubMed: 25127069|
|J Mol Cell Cardiol||Diverse contribution of bone marrow-derived late-outgrowth endothelial progenitor cells to vascular repair under pulmonary arterial hypertension and arterial neointimal formation PubMed: 26231083|
|Endocrine||Effects of glucagon-like peptide-1 on advanced glycation endproduct-induced aortic endothelial dysfunction in streptozotocin-induced diabetic rats: possible roles of Rho kinase- and AMP kinase-mediated nuclear factor κB signaling pathways Pubmed:26758998|
|Mol Neurobiol||A Therapeutic Insight of Niacin and Coenzyme Q10 Against Diabetic Encephalopathy in Rats Pubmed:26867655|
|Pakistan journal of pharmaceutical sciences||Changes in blood pressure, vascular reactivity and inflammatory biomarkers following consumption of heated corn oil. pubmed:29084680|
|Acta Biochim Biophys Sin||Therapeutic potential of mesenchymal stem cells in acute kidney injury is affected by administration timing pubmed:28338909|
|Experimental and Therapeutic Medicine||Epigallocatechin‑3‑gallate attenuates neointimal hyperplasia in a rat model of carotid artery injury by inhibition of high mobility group box 1 expression pubmed:28962112|
|Experimental Parasitology||Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis pubmed:28821422|
|Evidence-Based Complementary and Alternative Medicine||BuShenKangShuai tablet reduces atherosclerotic lesion by improving blood lipids metabolism and inhibiting inflammatory response via TLR4 and NF-κB signaling pathway journals:1758383|
|Journal of Chromatography A||Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis S0021967317311858|
|International Journal of Clinical and Experimental Pathology||Protective effect of high mobility group box-1 silence on diabetic retinopathy: an in vivo study ISSN:1936-2625/IJCEP0055881|
|ResearchGate||Significance of Soluble Endoglin as a Possible Marker of Cardiovascular Disease in Type 2 Diabetic Patients 315113405|
|Regenerative Biomaterials||The directional migration and differentiation of mesenchymal stem cells toward vascular endothelial cells stimulated by biphasic calcium phosphate ceramic 10.1093/rb/rbx028|
|Anesthesiology||Vasculotide, an angiopoietin-1 mimetic, restores microcirculatory perfusion and microvascular leakage and decreases fluid resuscitation requirements in … Pubmed:28968277|
|PLoS Neglected Tropical Diseases||Characterization of the rhesus macaque (Macaca mulatta) scrub typhus model: Susceptibility to intradermal challenge with the human pathogen Orientia … Pubmed:29522521|
|Life Sciences||Nephroprotective effect of saxagliptin against gentamicin-induced nephrotoxicity, emphasis on anti-oxidant, anti-inflammatory and anti-apoptic effects Pubmed:30012474|
|Evidence-based Complementary and Alternative Medicine||Bushenkangshuai Tablet Reduces Atherosclerotic Lesion by Improving Blood Lipids Metabolism and Inhibiting Inflammatory Response via TLR4 and NF-κB Signaling … Pubmed:29619063|
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