ELISA Kit for Enolase, Neuron Specific (NSE)

ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase

Specificity

This assay has high sensitivity and excellent specificity for detection of Enolase, Neuron Specific (NSE).
No significant cross-reactivity or interference between Enolase, Neuron Specific (NSE) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Enolase, Neuron Specific (NSE) and the recovery rates were calculated by comparing the measured value to the expected amount of Enolase, Neuron Specific (NSE) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-92 88
EDTA plasma(n=5) 91-99 96
heparin plasma(n=5) 96-103 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Enolase, Neuron Specific (NSE) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Enolase, Neuron Specific (NSE) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Enolase, Neuron Specific (NSE) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-103% 81-101% 82-94% 93-101%
EDTA plasma(n=5) 94-101% 96-103% 83-92% 98-105%
heparin plasma(n=5) 95-105% 83-91% 93-101% 94-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Neurotrauma The Anti-Inflammatory and Neuroprotective Effects of Ghrelin in Subarachnoid Hemorrhage-Induced Oxidative Brain Damage in Rats Liebert: 20091210
20 Melatonin treatment protects against spinal cord injury induced functional and biochemical changes in rat urinary bladder Wiley: source
Journal of Pharmacology and Toxicology Ginkgo biloba Enhances the Anticonvulsant and Neuroprotective Effects of Sodium Valproate Against Kainic Acid-induced Seizures in Mice Medwell: 2011679690
Addiction Biology Comparative gene expression profiling analysis of lymphoblastoid cells reveals neuron‐specific enolase gene (ENO2) as a susceptibility gene of heroin dependence PubMed: 21995595
Placenta Advanced glycation end products as an upstream molecule triggers ROS-induced sFlt-1 production in extravillous trophoblasts: A novel bridge between oxidative stress and preeclampsia Pubmed: 24144948
J Ethnopharmacol. Therapeutic time window and underlying therapeutic mechanism of breviscapine injection against cerebral ischemia/reperfusion injury in rats. Pubmed: 24291152
J Neurosurg. Detection of β-amyloid oligomers as a predictor of neurological outcome after brain injury. Pubmed: 23540266
Analytical Methods A portable and quantitative enzyme immunoassay of neuron-specific enolase with a glucometer read out Rsc: Source
Int J Neurosci. The effect of therapeutic hypothermia after cardiopulmonary resuscitation on ICAM-1 and NSE levels in sudden cardiac arrest rabbits Pubmed:25111247
Experimental &amp; Molecular Medicine Elevated levels of serum sICAM-1 in asphyxiated low birth weight newborns Pubmed:25358349
Neuroscience Letters Preconditioning effect of (S)-3,5-dihydroxyphenylglycine on ischemic injury in middle cerebral artery occluded Sprague-Dawley rats. Pubmed:25562631
中华临床医师杂志 高迁移率族蛋白B1和神经元烯醇化酶对心脏骤停大鼠复苏后脑损伤评价的研究 Journal: Fulltext
Am J Trop Med Hyg Blood–Brain Barrier Function and Biomarkers of Central Nervous System Injury in Rickettsial versus Other Neurological Infections in Laos PubMed: 26055741
Neurosci Lett Preconditioning effect of (S)-3, 5-dihydroxyphenylglycine on ischemic injury in middle cerebral artery occluded Sprague–Dawley rats PubMed: 25562631
Physiol Rep S100B and NSE serum concentrations after simulated diving in rats PubMed: 26462746
Int J Mol Sci The effect of electroporation of a lyotroic liquid crystal Genistein-based formulation in the recovery of murine melanoma lesions PubMed: 26184156
PLoS One Isoflurane Post-Treatment Improves Outcome after an Embolic Stroke in Rabbits PubMed: 26645542
Фармакология, клиническая фармакология Волгоградский государственный медицинский университет uploads:dsovet
Analytical Methods Synthesis of sunflower-like gold nanostructures and their application in electrochemical immunoassays using the nanogold-triggered hydrogen evolution reaction articlelanding:c5ay03174e
中国临床药理学与治疗学 右美托咪定对脑膜瘤切除术患者血清S100β 蛋白及神经元特异性烯醇化酶的影响 article:9794
Asian Pacific Journal of Tropical Medicine Perfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injury science:S1995764516300955
Izvorni znanstveni članak SERUMSKA RAZINA ZA NEURON SPECIFIČNE ENOLAZE KAO BILJEG ISHEMIJSKO-REPERFUZIJSKOG OŠTEĆENJA U BOLESNIKA PODVRGNUTIH KAROTIDNOJ ENDARTEREKTOMIJI 262238
Neurourol Urodyn Functional and structural changes of the urinary bladder following spinal cord injury; treatment with alpha lipoic acid pubmed:27490041
Biomedicine & Pharmacotherapy Rosuvastatin improves myocardial and neurological outcomes after asphyxial cardiac arrest and cardiopulmonary resuscitation in rats pubmed:28076830
cellular physiology and biochemistry Hydrogen-Rich Saline Attenuates Brain Injury Induced by Cardiopulmonary Bypass and Inhibits Microvascular Endothelial Cell Apoptosis Via the PI3K/Akt/GSK3β … pubmed:29040978
Brain Research Intra-arterial human urinary kallidinogenase alleviates brain injury in rats with permanent middle cerebral artery occlusion through PI3K/AKT/FoxO1 signaling pathway Pubmed:29510144
Biomed Research International Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway Pubmed:29770336
Journal of the Neurological Sciences S100B, NSE and MMP-9 fail to predict neurologic outcome while elevated S100B associates with milder initial clinical presentation after aneurysmal subarachnoid … Pubmed:29801873
Iranian Journal of Veterinary Research Nitrosative stress indices in dogs with neurological form of canine distemper Doi: 10.22099/IJVR.2018.4943
Evidence-Based Complementary and Alternative Medicine Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3K/PDK1/AKT Signaling Pathway
The Eurasian Journal of Medicine Effects of Conivaptan versus Mannitol on Post-Ischemic Brain Injury and Edema
Medicina The Administration of the New Pyrimidine Derivative—4-{2-[2-(3, 4-Dimethoxyphenyl)-Vinyl]-6-Ethyl-4-Oxo-5-Phenyl-4H-Pyrimidine-1-Il} Benzsulfamide Restores the …
ACTA NEUROBIOLOGIAE EXPERIMENTALIS Neuroprotective effect of poly (lactic-co-glycolic acid) nanoparticle-bound brain-derived neurotrophic factor in a permanent middle cerebral artery occlusion … Pubmed: 32214270
Stem Cells International Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 … Pubmed: 32300366
European Review for Medical and Pharmacological Sciences Neuroprotective effect of CTRP3 overexpression against sevoflurane anesthesia-induced cognitive dysfunction in aged rats through activating AMPK/SIRT1 and … Pubmed: 32432773
Neurobiology of Learning and Memory Effects of Circadian Rhythm Disorder on the Hippocampus of SHR and WKY Rats
The role of light desynchronosis in the development of stress-induced aging
ИЗУЧЕНИЕ ЦЕРЕБРОПРОТЕКТОРНОГО ДЕЙСТВИЯ ПРЕПАРАТОВ РАЗЛИЧНЫХ ФАРМАКОЛОГИЧЕСКИХ ГРУПП В УСЛОВИЯХ …
Chinese Journal of Analytical Chemistry Investigation of Library Input on Aptamers Selection Efficiency Using Capillary Electrophoresis
Ther Adv Chronic Dis The effect of doxycycline on neuron-specific enolase in patients with traumatic brain injury: a randomized controlled trial 34262678
J Surg Res Perillaldehyde Alleviates Spinal Cord Ischemia-Reperfusion Injury Via Activating the Nrf2 Pathway 34399353
Cephalalgia Evaluation of serum neuron specific enolase levels among patients with primary and secondary burning mouth syndrome 34644195
Catalog No. Related products for research use of Rhesus monkey (Simian) Organism species Applications (RESEARCH USE ONLY!)
SEA537Si ELISA Kit for Enolase, Neuron Specific (NSE) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA537Si Multiplex Assay Kit for Enolase, Neuron Specific (NSE) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.