ELISA Kit for Thrombomodulin (TM)
CD141; THBD; BDCA3; THRM; Fetomodulin
- Product No.SEA529Rb
- Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.059ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 684
For more details, please contact local distributors! US$ 3078
For more details, please contact local distributors! US$ 5814
For more details, please contact local distributors! US$ 47880
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Thrombomodulin (TM).
No significant cross-reactivity or interference between Thrombomodulin (TM) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Thrombomodulin (TM) and the recovery rates were calculated by comparing the measured value to the expected amount of Thrombomodulin (TM) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thrombomodulin (TM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thrombomodulin (TM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Thrombomodulin (TM) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|PLoS One||A Non-Lethal Traumatic/Hemorrhagic Insult Strongly Modulates the Compartment-Specific PAI-1 Response in the Subsequent Polymicrobial Sepsis PubMed: PMC3568129|
|Clin Appl Thromb Hemost.||Is There Any Association Between Thrombosis and Tissue Factor Pathway Inhibitor Levels in Patients With Vitamin D Deficiency? Pubmed: 24203351|
|медицинские науки||КЛЕТОЧНО-МОЛЕКУЛЯРНЫЕ МАРКЕРЫ ЭНДОТЕЛИАЛЬНОЙ ДИСФУНКЦИИ И СИСТЕМНОГО ГЕМОСТАЗА ПРИ ВИБРАЦИОННОЙ БОЛЕЗНИ В … Ru:Source|
|FASEB J.||Endothelial mineralocorticoid receptor activation enhances endothelial protein C receptor and decreases vascular thrombosis in mice Pubmed:24451386|
|Gynecol Endocrinol.||The effect of hormone therapy on biochemical and ultrasound parameters associated with atherosclerosis in 46, XY DSD individuals with female phenotype Pubmed:24911331|
|Informa Healthcare||Changes in cardiac thrombomodulin and heat shock transcription factor 1 expression and peripheral thrombomodulin and catecholamines during hypothermia in rats Pubmed:25109347|
|Bone Marrow Transplant.||Thrombomodulin alleviates murine GVHD in association with an increase in the proportion of regulatory T cells in the spleen Pubmed:25243628|
|Biomed Res Int||Basic Fibroblast Growth Factor Ameliorates Endothelial Dysfunction in Radiation-Induced Bladder Injury PubMed: 26351640|
|Molecular Nutrition & Food Research||Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors PubMed: 25546502|
|Acta Biomater||Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture study PubMed: 26689470|
|MEDICINE AND EDUCATION IN SIBERIA||КЛЕТОЧНО-МОЛЕКУЛЯРНЫЕ МАРКЕРЫ ЭНДОТЕЛИАЛЬНОЙ ДИСФУНКЦИИ И СИСТЕМНОГО ГЕМОСТАЗА ПРИ ВИБРАЦИОННОЙ БОЛЕЗНИ В СОЧЕТАНИИ С АРТЕРИАЛЬНОЙ ГИПЕРТЕНЗИЕЙ Cozo: Mos|
|Acta Biomaterialia||Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture study Pubmed:26689470|
|Bone Marrow Transplantation||The fifth epidermal growth factor-like region of thrombomodulin exerts cytoprotective functionand prevents SOS in a murine model. pubmed:27427915|
|BMC Vet Res.||Blood markers of fibrinolysis and endothelial activation in canine babesiosis. pubmed:28363279|
|Haematologica||Cytoprotective and pro-angiogenic functions of thrombomodulin are preserved in the C loop of the fifth epidermal growth factor-like domain Pubmed:29903766|
|Biomedicine & Pharmacotherapy||Puerarin prevents vascular endothelial injury through suppression of NF-κB activation in LPS-challenged human umbilical vein endothelial cells Pubmed:29775893|
|Catalog No.||Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA529Rb01||Recombinant Thrombomodulin (TM)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA529Rb51||Polyclonal Antibody to Thrombomodulin (TM)||WB; IHC; ICC; IP.|
|MAA529Rb21||Monoclonal Antibody to Thrombomodulin (TM)||WB; IHC; ICC; IP.|
|SEA529Rb||ELISA Kit for Thrombomodulin (TM)||Enzyme-linked immunosorbent assay for Antigen Detection.|