ELISA Kit for Oxidized Low Density Lipoprotein (OxLDL)



This assay has high sensitivity and excellent specificity for detection of Oxidized Low Density Lipoprotein (OxLDL).
No significant cross-reactivity or interference between Oxidized Low Density Lipoprotein (OxLDL) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Oxidized Low Density Lipoprotein (OxLDL) and the recovery rates were calculated by comparing the measured value to the expected amount of Oxidized Low Density Lipoprotein (OxLDL) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-96 92
EDTA plasma(n=5) 99-105 102
heparin plasma(n=5) 79-103 101


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Oxidized Low Density Lipoprotein (OxLDL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Oxidized Low Density Lipoprotein (OxLDL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Oxidized Low Density Lipoprotein (OxLDL) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-102% 88-98% 83-93% 90-97%
EDTA plasma(n=5) 92-102% 90-104% 90-99% 91-99%
heparin plasma(n=5) 85-103% 92-105% 88-95% 90-97%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
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Vascular Medicine Anti‐Inflammatory Immune Skewing Is Atheroprotective: Apoe−/−FcγRIIb−/− Mice Develop Fibrous Carotid Plaques Pubmed:25516435
Journal of Functional Foods Oral administration of the β-glucan produced by Aureobasidium pullulans ameliorates development of atherosclerosis in apolipoprotein E deficient mice Science: Article
Acta Pharmacologica Sinica Sirt1 is involved in decreased bone formation in aged apolipoprotein E-deficient mice PubMed: 26592520
Cancer Science Suppressive effects of the NADPH oxidase inhibitor apocynin on intestinal tumorigenesis in obese KK‐Ay and Apc mutant Min mice PubMed: 26310859
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PLoS One Ezetimibe Attenuates Atherosclerosis Associated with Lipid Reduction and Inflammation Inhibition PubMed: 26555472
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Diabetology & Metabolic Syndrome Oxidative DNA damage and oxidized low density lipoprotein in Type II diabetes mellitus among patients with Helicobacter pylori infection pmc:PMC4855822
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Journal of Applied Pharmaceutical Science Organic Arsenical Exposure Stimulates Atherosclerosis through Oxidative Stress Increase and Adhesion Molecule Expression admin:php
Journal of Nutritional Biochemistry Dietary rose hip exerts anti-atherosclerotic effects and increases nitric oxide-mediated dilation in ApoE-null mice S0955286316301887
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Journal of Functional Foods Atherosclerosis-related inflammation and oxidative stress are improved by rice bran enzymatic extract S1756464616302511
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biorxiv Significant metabolic improvement by a water extract of olives: animal and human evidence 10.1101/151860
Journal of nutritional biochemistry Dietary rose hip exerts antiatherosclerotic effects and increases nitric oxide-mediated dilation in ApoE-null mice. pubmed:28399420
Nutrients Yirui Capsules Alleviate Atherosclerosis by Improving the Lipid Profile and Reducing Inflammation in Apolipoprotein E-Deficient Mice Pubmed:29382111
Nutrition & Metabolism Cold-pressed minke whale oil reduces circulating LDL/VLDL-cholesterol, lipid oxidation and atherogenesis in apolipoprotein E-deficient mice fed a Western … Pubmed:29755576
Journal of Functional Foods Saponins of sea cucumber attenuate atherosclerosis in ApoE−/− mice via lipid-lowering and anti-inflammatory properties 10.1016:j.jff.2018.07.046
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Molecules Protective Effect of Polyphenol-Rich Extract from Bee Pollen in a High-Fat Diet Pubmed:29614743
Lipids in Health and Disease Lipingshu capsule improves atherosclerosis associated with lipid regulation and inflammation inhibition in apolipoprotein E–deficient mice Pubmed:30064511
Diabetes Metabolism Research and Reviews  Diabetic dyslipidaemia is associated with alterations in eNOS, caveolin‐1, and endothelial dysfunction in streptozotocin treated rats Pubmed:29471582
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