ELISA Kit for Inhibin Alpha (INHa)

INH-A

Specificity

This assay has high sensitivity and excellent specificity for detection of Inhibin Alpha (INHa).
No significant cross-reactivity or interference between Inhibin Alpha (INHa) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Inhibin Alpha (INHa) and the recovery rates were calculated by comparing the measured value to the expected amount of Inhibin Alpha (INHa) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-104 101
EDTA plasma(n=5) 80-103 98
heparin plasma(n=5) 80-91 85

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inhibin Alpha (INHa) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inhibin Alpha (INHa) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Inhibin Alpha (INHa) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 97-105% 85-92% 90-101% 91-99%
EDTA plasma(n=5) 80-97% 96-105% 85-97% 87-95%
heparin plasma(n=5) 98-105% 90-98% 80-105% 96-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Stem Cell Research & Therapy Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the … Pubmed: 30606243
Clinical and Experimental Obstetrics & Gynecology Biphasic Effect of TNF-α on the Survival and Endocrine Function of Human Ovarian Granulosa Cells
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA395Mu01 Recombinant Inhibin Alpha (INHa) Positive Control; Immunogen; SDS-PAGE; WB.
RPA395Mu02 Recombinant Inhibin Alpha (INHa) Positive Control; Immunogen; SDS-PAGE; WB.
PAA395Mu01 Polyclonal Antibody to Inhibin Alpha (INHa) WB; IHC; ICC; IP.
PAA395Mu02 Polyclonal Antibody to Inhibin Alpha (INHa) WB; IHC; ICC; IP.
SEA395Mu ELISA Kit for Inhibin Alpha (INHa) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA395Mu ELISA Kit for Inhibin Alpha (INHa) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA395Mu CLIA Kit for Inhibin Alpha (INHa) Chemiluminescent immunoassay for Antigen Detection.
LMA395Mu Multiplex Assay Kit for Inhibin Alpha (INHa) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA395Mu11 ELISA Kit DIY Materials for Inhibin Alpha (INHa) Main materials for "Do It (ELISA Kit) Yourself".