ELISA Kit for Collagen Type I Alpha 2 (COL1a2)

COL1-A2; OI4; Collagen Alpha-2(I)chain; osteogenesis imperfecta type IV; Collagen Of Skin,Tendon And bone,Alpha-2 Chain

Specificity

This assay has high sensitivity and excellent specificity for detection of Collagen Type I Alpha 2 (COL1a2).
No significant cross-reactivity or interference between Collagen Type I Alpha 2 (COL1a2) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Collagen Type I Alpha 2 (COL1a2) and the recovery rates were calculated by comparing the measured value to the expected amount of Collagen Type I Alpha 2 (COL1a2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-96 89
EDTA plasma(n=5) 97-105 102
heparin plasma(n=5) 82-105 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type I Alpha 2 (COL1a2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type I Alpha 2 (COL1a2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Collagen Type I Alpha 2 (COL1a2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-102% 78-94% 97-104% 92-101%
EDTA plasma(n=5) 96-105% 79-98% 83-96% 82-98%
heparin plasma(n=5) 88-102% 78-98% 83-97% 90-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Plos one Inhibition of TGF-β Signaling Enables Human Corneal Endothelial Cell Expansion In Vitro for Use in Regenerative Medicine PubMed: PMC3581499
J Proteomics Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC) PubMed: 26718731
PLoS One Pro-fibrotic compounds induce stellate cell activation, ECM-remodelling and Nrf2 activation in a human 3D-multicellular model of liver fibrosis pubmed:28665955
American Journal of Translational Research A novel hybrid 3D-printed titanium scaffold for osteogenesis in a rabbit calvarial defect model Pubmed:29511441
Sci Rep The effect of hypoxia on the proteomic signature of pig adipose-derived stromal/stem cells (pASCs) Pubmed: 33208768
Experimental and Therapeutic Medicine Protective effects of halophyte complex extract against UVB?induced damage in human keratinocytes and the skin of hairless mice 33986847
Catalog No. Related products for research use of Canis familiaris; Canine (Dog) Organism species Applications (RESEARCH USE ONLY!)
RPA215Ca01 Recombinant Collagen Type I Alpha 2 (COL1a2) Positive Control; Immunogen; SDS-PAGE; WB.
PAA215Ca01 Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2) WB; IHC; ICC; IP.
MAA215Ca21 Monoclonal Antibody to Collagen Type I Alpha 2 (COL1a2) WB; IHC; ICC; IP.
SEA215Ca ELISA Kit for Collagen Type I Alpha 2 (COL1a2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA215Ca Multiplex Assay Kit for Collagen Type I Alpha 2 (COL1a2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.