ELISA Kit for Pyruvate kinase isozymes R/L (PKLR)

PK; PyK; PK1; PKL; Pyruvate Kinase Isozymes R/L; Glutamic--pyruvic transaminase 1; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase

Specificity

This assay has high sensitivity and excellent specificity for detection of Pyruvate kinase isozymes R/L (PKLR).
No significant cross-reactivity or interference between Pyruvate kinase isozymes R/L (PKLR) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Pyruvate kinase isozymes R/L (PKLR) and the recovery rates were calculated by comparing the measured value to the expected amount of Pyruvate kinase isozymes R/L (PKLR) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-97 88
EDTA plasma(n=5) 87-98 93
heparin plasma(n=5) 94-105 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pyruvate kinase isozymes R/L (PKLR) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pyruvate kinase isozymes R/L (PKLR) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pyruvate kinase isozymes R/L (PKLR) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-89% 80-92% 78-102% 82-90%
EDTA plasma(n=5) 89-99% 96-103% 96-105% 96-105%
heparin plasma(n=5) 94-105% 87-95% 88-104% 79-89%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Onco Targets Ther The effects of PGC-1α on the proliferation and energy metabolism of malignant endometrial cancer cells PubMed: 25914546
european journal of endocrinology Contact system activation and high thrombin generation in hyperthyroidism. pubmed:28137735
CELLULAR AND MOLECULAR NEUROBIOLOGY Time-Dependent Changes in the Serum Levels of Neurobiochemical Factors After Acute Methadone Overdose in Adolescent Male Rat Pubmed: 32712727
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA208Ra01 Recombinant Pyruvate kinase isozymes R/L (PKLR) Positive Control; Immunogen; SDS-PAGE; WB.
PAA208Ra01 Polyclonal Antibody to Pyruvate kinase isozymes R/L (PKLR) WB; IHC; ICC; IP.
MAA208Ra21 Monoclonal Antibody to Pyruvate kinase isozymes R/L (PKLR) WB; IHC; ICC; IP.
SEA208Ra ELISA Kit for Pyruvate kinase isozymes R/L (PKLR) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA208Ra Multiplex Assay Kit for Pyruvate kinase isozymes R/L (PKLR) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.