ELISA Kit for Plasminogen Activator, Urokinase Receptor (uPAR)

CD87; PLAUR; PLAU-R; U-PAR; URKR; Monocyte Activation Antigen Mo3

Specificity

This assay has high sensitivity and excellent specificity for detection of Plasminogen Activator, Urokinase Receptor (uPAR).
No significant cross-reactivity or interference between Plasminogen Activator, Urokinase Receptor (uPAR) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Plasminogen Activator, Urokinase Receptor (uPAR) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Plasminogen Activator, Urokinase Receptor (uPAR) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
International Journal of Cancer Dietary supplementation with methylseleninic acid, but not selenomethionine, reduces spontaneous metastasis of Lewis lung carcinoma in mice Wiley: source
Biomark Insights Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism PubMed: PMC3623607
Biomarker insights Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) PubMed: PMC3762604
Am J Emerg Med Clinical value of soluble urokinase-type plasminogen activator receptor in the diagnosis, prognosis, and therapeutic guidance of sepsis PubMed: 26615223
Archives of Clinical Infectious Diseases Evaluation of Diagnostic Value of Soluble Urokinase-Type Plasminogen Activator Receptor in Sepsis Archcid: 26346
The American Journal of Emergency Medicine Clinical value of soluble urokinase-type plasminogen activator receptor in the diagnosis, prognosis, and therapeutic Cavia (Guinea pig )idance of sepsis science:S0735675715009559
Journal of Clinical and Experimental Hepatology Evaluation of Plasma Urokinase-Type Plasminogen Activator Receptor (UPAR) in Patients With Chronic Hepatitis B, C and Non-Alcoholic Fatty Liver Disease (NAFLD) … 10.1016:j.jceh.2018.02.001
Turkish journal of trauma & emergency surgery The diagnostic value of serum urokinase-type plasminogen activator receptor in acute appendicitis Pubmed: 31475329
CELL TRANSPLANTATION Predictive Value of Degranulating Factors of Neutrophils in Massive Cerebral Infarction 33787356
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA141Hu01 Recombinant Plasminogen Activator, Urokinase Receptor (uPAR) Positive Control; Immunogen; SDS-PAGE; WB.
RPA141Hu02 Recombinant Plasminogen Activator, Urokinase Receptor (uPAR) Positive Control; Immunogen; SDS-PAGE; WB.
APA141Hu04 Active Plasminogen Activator, Urokinase Receptor (uPAR) Cell culture; Activity Assays.
RPA141Hu04 Recombinant Plasminogen Activator, Urokinase Receptor (uPAR) Positive Control; Immunogen; SDS-PAGE; WB.
RPA141Hu03 Recombinant Plasminogen Activator, Urokinase Receptor (uPAR) Positive Control; Immunogen; SDS-PAGE; WB.
PAA141Hu04 Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR) WB
PAA141Hu01 Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR) WB; IHC; ICC; IP.
PAA141Hu02 Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR) WB; IHC; ICC; IP.
PAA141Hu03 Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR) WB; IHC; ICC; IP.
LAA141Hu71 Biotin-Linked Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR) WB; IHC; ICC.
LAA141Hu83 FITC-Linked Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR) WB; IHC; ICC; IF.
LAA141Hu81 FITC-Linked Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR) WB; IHC; ICC; IF.
FAA141Hu81 FITC-Linked Anti-Plasminogen Activator, Urokinase Receptor (uPAR) Polyclonal Antibody FCM
MAA141Hu22 Monoclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR) WB; IHC; ICC; IP.
MAA141Hu21 Monoclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR) WB; IHC; ICC; IP.
LAA141Hu82 FITC-Linked Monoclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR) WB; IHC; ICC; IF.
SEA141Hu ELISA Kit for Plasminogen Activator, Urokinase Receptor (uPAR) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA141Hu Multiplex Assay Kit for Plasminogen Activator, Urokinase Receptor (uPAR) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.