ELISA Kit for Osteoprotegerin (OPG)
TNFRSF11B; TNFRSF11-B; OCIF; OPG; TR1; Tumor Necrosis Factor Receptor Superfamily Member 11b; Osteoclastogenesis Inhibitory Factor
- Product No.SEA108Po
- Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.059ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Osteoprotegerin (OPG).
No significant cross-reactivity or interference between Osteoprotegerin (OPG) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Osteoprotegerin (OPG) and the recovery rates were calculated by comparing the measured value to the expected amount of Osteoprotegerin (OPG) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osteoprotegerin (OPG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteoprotegerin (OPG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Osteoprotegerin (OPG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Pharmacology||Protective Effect of Amlodipine on Rat Bone Tissue after Orchidectomy Karger: 335491|
|Endocrine Pharmacology||Protective effect of atorvastatin on bone tissue in orchidectomised male albino Wistar rats ScienceDirect: S0014299912000659|
|PLOS One||Adipokines in Psoriatic Arthritis Patients: The Correlations with Osteoclast Precursors and Bone Erosions PubMed: PMC3483160|
|International Journal of Oral and Maxillofacial Surgery||The effects of chronic zoledronate usage on the jaw and long bones evaluated using RANKL and osteoprotegerin levels in an animal model Pubmed: 23522850|
|American Journal of Orthodontics and Dentofacial Orthopedics||OPG and RANKL levels around miniscrew implants during orthodontic tooth movement Pubmed: 23910201|
|AGE||Estrogen deficiency does not decrease the in vitro osteogenic potential of rat adipose-derived mesenchymal stem cells Pubmed: 24687841|
|Food Science & Nutrition||Effects and mechanisms of 8-prenylnaringenin on osteoblast MC3T3-E1 and osteoclast-like cells RAW264.7 Onlinelibrary: fsn3.109|
|Food Sci Nutr.||Effects and mechanisms of 8‐prenylnaringenin on osteoblast MC3T3‐E1 and osteoclast‐like cells RAW264. 7 Pubmed:Pmc4221832|
|J Neurol Sci.||The effect of topiramate and lamotrigine on rat bone mass, structure and metabolism. Pubmed:24629477|
|Journal of Investigative Surgery||Effect of Nicotine on RANKL and OPG and Bone Mineral Density Pubmed:24830656|
|Int J Mol Med.||Effects of icariin on the regulation of the OPG?RANKL?RANK system are mediated through the MAPK pathways in IL?1β?stimulated human SW1353 chondrosarcoma … Pubmed:25270538|
|Clinical and Experimental Pharmacology and Physiology||Carvedilol promotes neurological function, reduces bone loss and attenuates cell damage after acute spinal cord injury in rats Pubmed:25424914|
|Journal of Colloid and Interface Science||Strontium and zoledronate hydroxyapatites graded composite coatings for bone prostheses Pubmed:25706198|
|Int J Clin Exp Pathol||A novel model of bisphosphonate-related osteonecrosis of the jaw in rats PubMed: 26191212|
|IJHS(international journal of health sciences)||Relation of Osteoprotegerin, Visfatin and Ghrelin to Metabolic Syndrome in Type 2 Diabetic Patients PubMed: 26309431|
|Pharmacology||Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy PubMed: 25871861|
|Experimental And Therapeutic Medicine||Antiosteoporotic effect of icariin in ovariectomized rats is mediated via the Wnt/β-catenin pathway Pubmed:27347050|
|Bone||The effects of strength training and raloxifene on bone health in aging ovariectomized rats Pubmed:26812611|
|Scienze Biomediche||Effetti del laser a bassa dose su cellule osteoblastiche INCERTIPARENTI_SERENA_TESI.pdf|
|journal of biomedical materials research part a||RAW 264.7 co-cultured with ultra-high molecular weight polyethylene particles spontaneously differentiate into osteoclasts: an in vitro model of periprosthetic osteolysis. pubmed:27667508|
|Nutrition.||Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats. pubmed:27908549|
|PLoS One.||Extracorporeal shock waves alone or combined with raloxifene promote bone formation and suppress resorption in ovariectomized rats. pubmed:28158228|
|Sci Rep.||Skeletal Site-specific Effects of Zoledronate on in vivo Bone Remodeling and in vitro BMSCs Osteogenic Activity. pubmed:28139685|
|Journal of Biomaterials and Tissue Engineering||Modification and Characterization of Polyethylene Glycolated Decellularized Porcine Aortic Heart Valve with Osteoprotegerin art00007|
|Japanese Society for Bone and Mineral Research||Allogeneic yet major histocompatibility complex‑matched bone marrow transplantation in mice results in an impairment of osteoblasts and a significantly reduced trabecular bone article:10.1007|
|ONCOLOGY LETTERS||Assessment of bone metabolism and biomechanical properties of the femur, following treatment with anastrozole and letrozole in an experimental model of menopause ol.2017.6596|
|Journal of Biomedical Materials Research||RAW 264.7 co-cultured with ultra-high molecular weight polyethylene particles spontaneously differentiate into osteoclasts: an in vitro model of periprosthetic osteolysis 10.1002/jbm.a.35912|
|Oncotarget||Mesenchymal stem cells inhibit RANK-RANKL interactions between osteoclasts and Th17 cells via osteoprotegerin activity. pubmed:29137353|
|Asian Journal of Biomedical and Pharmaceutical Sciences||A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/ÃÂ²-catenin Signaling Pathways in Ovariectomized Rats ISSN 2249 – 622X|
|ACS Applied Materials and Interfaces||Enhanced Osseointegration of Porous Titanium Modified with Zeolitic Imidazolate Framework-8. pubmed:28696091|
|Catalog No.||Related products for research use of Sus scrofa; Porcine (Pig) Organism species||Applications (RESEARCH USE ONLY!)|
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|PAA108Po01||Polyclonal Antibody to Osteoprotegerin (OPG)||WB; IHC; ICC; IP.|
|MAA108Po21||Monoclonal Antibody to Osteoprotegerin (OPG)||WB; IHC; ICC; IP.|
|SEA108Po||ELISA Kit for Osteoprotegerin (OPG)||Enzyme-linked immunosorbent assay for Antigen Detection.|