ELISA Kit for Interleukin 4 (IL4)
BSF1; BCGF1 ;B Cell Stimulatory Factor 1; Lymphocyte stimulatory factor 1; B Cell Growth Factor; Binetrakin; Pitrakinra
- Product No.SEA077Gu
- Organism SpeciesCavia (Guinea pig ) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range3.12-200pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.24pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 720
For more details, please contact local distributors! US$ 3240
For more details, please contact local distributors! US$ 6120
For more details, please contact local distributors! US$ 50400
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Interleukin 4 (IL4).
No significant cross-reactivity or interference between Interleukin 4 (IL4) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Interleukin 4 (IL4) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 4 (IL4) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 4 (IL4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 4 (IL4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 4 (IL4) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Int immunopharmacol||Validation of guinea pig model of allergic rhinitis by oral and topical drugs(guinea pig ) PubMed: 18625276|
|International Archives of Allergy and Immunology||Characterization of Anti-Inflammatory Properties and Evidence for No Sedation Liability for the Novel Antihistamine SUN-1334H PubMed: 19672097|
|Molecular Biology Reports||Dexamethasone attenuates development of monocrotaline-induced pulmonary arterial hypertension SpringerLink: 76255j50271l4nh2|
|6th International Scientific Interdisciplinary Conference (ISIC)||Inflammation influence on the formation of endothelial dysfunction in hypertensive patients caused by obesity Knmu: Source|
|Theriogenology||Evaluation of serum concentrations of interleukin (IL)-4, IL-10, and IL-12 during pregnancy in bitches ScienceDirect: S0093691X1300037X|
|International Journal of Poultry Science||Immune Responses to Eimeria tenella Sporozoite Protein as Vaccine to Broiler Against Coccidiosis Pjbs: Source|
|European Journal of Pharmacology||Protective effects of neferine on amiodarone-induced pulmonary fibrosis in mice Pubmed: 23792144|
|Journal of Dairy Science||Increased blood-circulating interferon-γ, interleukin-17, and osteopontin levels in bovine paratuberculosis Pubmed:24679935|
|Biomedical Research||The toxic effect of nickel nanoparticles on oxidative stress and inflammatory markers. Articles:|
|Mol Med Rep||Roles of lipoxin A4 receptor activation and anti-interleukin-1β antibody on the toll-like receptor 2/mycloid differentiation factor 88/nuclear factor-κB pathway in airway inflammation induced by ovalbumin PubMed: 25760938|
|veterinary research forum||Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells Article_14936_6.Html:|
|Immunologic Research||Mesenchymal stromal cell-dependent reprogramming of Kupffer cells is mediated by TNF-α and PGE3 and is crucial for liver transplant tolerance PubMed: 25982496|
|J Reprod Immunol||The immunomodulating effect of seminal plasma on T cells PubMed: 25799173|
|Arch Virol||Analysis of cytokine production in a newly developed canine tracheal epithelial cell line infected with H3N2 canine influenza virus PubMed: 25797195|
|удк||Циток Cgi-Bin: Irbis_Nbuv|
|Vet Immunol Immunopathol||Immune responses of pigs immunized with a recombinant porcine reproductive and respiratory syndrome virus expressing porcine GM-CSF PubMed: 26300317|
|Virol J||Rescue and evaluation of a recombinant PRRSV expressing porcine Interleukin-4 PubMed: 26573719|
|Int J Clin Exp Pathol||Downregulation of SUMF2 gene in ovalbumin-induced rat model of allergic inflammation PubMed: 26722390|
|Infection, Genetics and Evolution||Infection of chicken bone marrow mononuclear cells with subgroup J avian leukosis virus inhibits dendritic cell differentiation and alters cytokine expression Pubmed:27349993|
|Plos One||Immunopotentiators Improve the Efficacy of Oil-Emulsion-Inactivated Avian Influenza Vaccine in Chickens, Ducks and Geese Pubmed:27232188|
|Molecular Immunology||Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by reCavia (Guinea pig )lating caveolin-1 through TLR2/MyD88/NF-ŚĘB in murine model of asthma Pubmed:27289030|
|Mediators Inflamm||Proinflammatory and Anti-Inflammatory Cytokines Mediated by NF-ÎşB Factor as Prognostic Markers in Mammary Tumors Pubmed:26989335|
|Asian J Pharm Clin Res||INFLUENCE OF ZINCOVIT DROP (NUTRITIONAL FOOD SUPPLEMENT) ON IMMUNE SYSTEM IN NORMAL AND CYCLOPHOSPHAMIDE INTOXICATED WISTAR RATS journals:3790|
|Int J Clin Exp Pathol||Effects of Dectin-1 on the mast cells in allergic conjunctivitis and its underlying mechanism files:ijcep0019305.pdf|
|BMC Veterinary Research||Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis pubmed:27553600|
|immunology letters||Recombinant Mip-PilE-FlaA dominant epitopes vaccine candidate against Legionella pneumophila. pubmed:28366526|
|Antiviral research||Evaluation of two strains of Marek's disease virus serotype 1 for the development of recombinant vaccines against very virulent infectious bursal disease virus. pubmed:27908832|
|Vaccine.||Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J. pubmed:28049588|
|American Journal of Translational Research||Anti-inflammatory effects of Boletus edulis polysaccharide on asthma pathology pubmed:27830033|
|Molecular Immunology||Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by regulating caveolin-1 through TLR2/MyD88/NF-κB in murine model of asthma pubmed:27289030|
|Basrah Journal of Veterinary Research||EVALUATION OF HUMORAL AND CELLULAR IMMUNE RESPONSES TO EIMERIA TENELLA OOCYST PROTEIN AS VACCINE TO BROILER 363-379.pdf|
|Mediators of Inflammation||Proinflammatory and Anti-Inflammatory Cytokines Mediated by NF-κB Factor as PrognosticMarkers in Mammary Tumors. pubmed:26989335|
|Sci Rep.||Long-term use of ceftriaxone sodium induced changes in gut microbiota and immune system. pubmed:28220870|
|Acta Vet Hung.||Imbalance of intestinal immune function in piglets infected by porcine circovirus type 2 during the fetal period. pubmed:28244338|
|Veterinary Immunology and Immunopathology||Cytokine levels in colostrum and in foals' serum pre- and post-suckling pubmed:28242000|
|Catalog No.||Related products for research use of Cavia (Guinea pig ) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA077Gu01||Recombinant Interleukin 4 (IL4)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA077Gu01||Polyclonal Antibody to Interleukin 4 (IL4)||WB; IHC; ICC; IP.|
|MAA077Gu21||Monoclonal Antibody to Interleukin 4 (IL4)||WB; IHC; ICC; IP.|
|SEA077Gu||ELISA Kit for Interleukin 4 (IL4)||Enzyme-linked immunosorbent assay for Antigen Detection.|