ELISA Kit for Interleukin 17 (IL17)
IL17A; CTLA8; IL-17A; Cytotoxic T-Lymphocyte-Associated Protein 8
- Product No.SEA063Bo
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range7.8-500pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 3.0ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Interleukin 17 (IL17).
No significant cross-reactivity or interference between Interleukin 17 (IL17) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Interleukin 17 (IL17) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 17 (IL17) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 17 (IL17) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|PLOS ONE||Impaired Autoimmune T Helper 17 Cell Responses Following DNA Vaccination against Rat Experimental Autoimmune Encephalomyelitis PubMed: PMC2577303|
|Cellular Immunology||Atorvastatin ameliorates experimental autoimmune neuritis by decreased Th1/Th17 cytokines and up-regulated T regulatory cells ScienceDirect: S0008874911002164|
|The Journal of Urology||Improvement by Phytotherapeutic Agent of Detrusor Overactivity, Down-Regulation of Pharmacological Receptors and Urinary Cytokines in Rats with Cyclophosphamide Induced Cystitis PubMed: S0022534712049087|
|Critical Care Medicine||Systemic release of cytokines and heat shock proteins in porcine models of polytrauma and hemorrhage PubMed: PMC3383056|
|Lower Urinary Tract Symptoms||Effects of Saw Palmetto Extract on Urodynamic Parameters, Bladder Muscarinic and Purinergic Receptors and Urinary Cytokines in Rats with Cyclophosphamide-Induced Cystitis Onlinelibrary: luts.12015|
|Shock||Expression of Hemopexin in Acute Rejection of Rat Liver Allograft Identified by Serum Proteomic Analysis. Lww: Source|
|J Vet Med Sci.||CD4+ T Cell Cytokine Gene and Protein Expression in Duodenal Mucosa of Dogs with Inflammatory Bowel Disease Pubmed:24270804|
|Journal of Dairy Science||Increased blood-circulating interferon-γ, interleukin-17, and osteopontin levels in bovine paratuberculosis Pubmed:24679935|
|Inflammation.?||Pemetrexed Ameliorates Experimental Arthritis in Rats Pubmed:25129058|
|Inflammation||Lapatinib Ameliorates Experimental Arthritis in Rats Pubmed:25283886|
|Molecular and Cellular Biochemistry||Beta-arrestin-2 negatively modulates inflammation response in mouse chondrocytes induced by 4-mer hyaluronan oligosaccharide Pubmed:25318610|
|Molecular Medicine Reports||Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosis Pubmed:25323532|
|Genet Mol Res||Protective effect and mechanism of probucol in the treatment of spinal cord injury PubMed: 26214485|
|veterinary research forum||Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells Article_14936_6.Html:|
|Tumor Biology||Hypoxia-induced secretion of IL-20 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma PubMed: 26695151|
|Clinical & Experimental Immunology||MicroRNA‐155 may be involved in the pathogenesis of atopic dermatitis by modulating the differentiation and function of T helper type 17 (Th18) cells PubMed: 25761610|
|J Reprod Immunol||The immunomodulating effect of seminal plasma on T cells PubMed: 25799173|
|Biomarkers||Interleukin-17 levels correlate with poor prognosis and vascular endothelial growth factor concentration in the serum of patients with non-small cell lung cancer PubMed: 26301881|
|Molecular Medicine Reports||Tanshinone IIA attenuates experimental autoimmune encephalomyelitis in rats Pubmed:27357729|
|SpringerPlus||Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression ofHMGB1/RAGE/NF-ΚB pathway. Pubmed:27026909|
|International Immunopharmacology||Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery Pubmed:26802602|
|Journal of Agricultural Biotechnology||Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells 10610|
|Veterinary Research Communications||Immunological and pathological investigations in equine experimental uveitis pubmed:27344152|
|Journal of Neuroinflammation||Th17-skewed immune response and cluster of differentiation 40 ligand expression in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis. pubmed:28114998|
|American Journal of Translational Research||Anti-inflammatory effects of Boletus edulis polysaccharide on asthma pathology pubmed:27830033|
|Springerplus||Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression of HMGB1/RAGE/NF-ΚB pathway pubmed:27026909|
|Journal of Virology||Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys. pubmed:|
|J Nephropathol||Relationship between IL-17 serum level and ambulatory blood pressure in women with polycystic ovary syndrome pubmed:28042549|
|Journal of Nephropathology||Relationship between IL-17 and ambulatory blood pressure in polycystic ovary syndrome 8b168a09f5832df1b5c2f48631612458b2d8.pdf|
|Basic Clin Pharmacol Toxicol.||Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling. pubmed:28032440|
|Gut Pathog.||Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice pubmed:28250847|
|Procalcitonin und proinflammatorische Zytokine in der bronchoalveolären Lavage beim lungenkranken Pferd unter inhalativer Glukokortikoidtherapie FUDISS_thesis_000000103830|
|Scientific Report||Anti-asthmatic efect of pitavastatin through aerosol inhalation is associated with CD4+ CD25+ Foxp3+ T cells in an asthma mouse model pubmed:28729731|
|Basic & Clinical Pharmacology & Toxicology||Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling doi:10.1111|
|Academic Journal||Antiinflammatory and antioxidant effects of gemcitabine in collagen-induced arthritis model pubmed:28618762|
|International Journal of Clinical and Experimental Pathology||Low-density neutrophils in severe fever with thrombocytopenia syndrome (SFTS) display decreased function to phagocytose SFTS virus and enhanced capacity to synthesize cytokines ISSN:1936-2625/IJCEP0045334|
|Asian Pacific Society of Respirology||Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice pubmed:28122397|
|MYOPAIN||Increased Levels of IL-17, IL-23, MIP-1α, MCP-1 and Global Leukocytes in Fibromyalgia Patients 10.1080/24708593.2017.1357664|
|Molecular Medicine Reports||Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia. pubmed:29257310|
|Molecular Medicine Reports||Interleukin-17 antagonist attenuates lung inflammation through inhibition of the ERK1/2 and NF-κB pathway in LPS-induced acute lung injury 10.3892/mmr.2017.6837|
|ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY||Dexamethasone reduces serum level of IL-17 in Bleomycin-A5-induced rats model of pulmonary fibrosis pubmed:28608724|
|International Immunopharmacology||Therapeutic effect of bone marrow mesenchymal stem cells pretreated with acetylsalicylic acid on experimental periodontitis in rats pubmed:29195233|
|Biomedical Research||Associations among interleukin-17, interferon-gamma, and acute coronary syndrome ISSN 0970-938X|
|Catalog No.||Related products for research use of Bos taurus; Bovine (Cattle) Organism species||Applications (RESEARCH USE ONLY!)|
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|MAA063Bo21||Monoclonal Antibody to Interleukin 17 (IL17)||WB; IHC; ICC; IP.|
|SEA063Bo||ELISA Kit for Interleukin 17 (IL17)||Enzyme-linked immunosorbent assay for Antigen Detection.|