ELISA Kit for Interferon Alpha (IFNa)
IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D
- Product No.SEA033Si
- Organism SpeciesRhesus monkey (Simian) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 6.4pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 810
For more details, please contact local distributors! US$ 3645
For more details, please contact local distributors! US$ 6885
For more details, please contact local distributors! US$ 56700
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Interferon Alpha (IFNa).
No significant cross-reactivity or interference between Interferon Alpha (IFNa) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Interferon Alpha (IFNa) and the recovery rates were calculated by comparing the measured value to the expected amount of Interferon Alpha (IFNa) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Alpha (IFNa) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Alpha (IFNa) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interferon Alpha (IFNa) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Journal of Animal and Veterinary Advances||Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease Vaccine Medwell: 2010610616|
|Journal of Animal and Veterinary Advances||Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease Virus Medwell: 2010594600|
|Ann Rheum Dis.||The cathelicidins LL-37 and rCRAMP are associated with pathogenic events of arthritis in humans and rats PubMed: 23172753|
|Nanomedicine||Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatform PubMed: 23394132|
|International journal of experimental pathology||Experimental rat model for alcohol-induced osteonecrosis of the femoral head PubMed: PMC3781777|
|Antiviral Research||A recombinant adenovirus bicistronically expressing porcine interferon-α and interferon-γ enhances antiviral effects against foot-and-mouth disease virus ScienceDirect: S0166354214000266|
|British Journal of Pharmacology||Andrographolide exerts anti-hepatitis C virus activity by up-regulating haeme oxygenase-1 via the p38 MAPK/Nrf2 pathway in human hepatoma cells Onlinelibrary: bph.12440|
|BMC Cancer||High efficiency of alphaviral gene transfer in combination with 5-fluorouracil in a mouse mammary tumor model Pubmed:24950740|
|PLoS One.||Respiratory Mechanics and Plasma Levels of Tumor Necrosis Factor Alpha and Interleukin 6 Are Affected by Gas Humidification during Mechanical Ventilation in Dogs Pubmed:25036811|
|Veterinary Research||Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strain Pubmed:25106750|
|Asian Pac J Trop Med||Effect of SecinH3 on lung injury induced by sepsis of rats PubMed: 26706678|
|PLoS One||siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In Vitro Pubmed:26886455|
|Plos One||Sulforaphane Suppresses Hepatitis C Virus Replication by Up-ReCavia (Guinea pig )lating Heme Oxygenase-1 Expressionthrough PI3K/Nrf2 Pathway. Pubmed:27023634|
|PLoS One.||Vaccination with recombinant Mycobacterium tuberculosis PknD attenuates bacterial dissemination to the brain in Cavia (Guinea pig )inea pigs. Pubmed:23776655|
|Veterinary microbiology||Impact of porcine reproductive and respiratory syndrome virus and porcine circovirus-2 infection on the potency of the classical swine fever vaccine (LOM strain). pubmed:27599928|
|International Journal of Clinical and Experimental Pathology||Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cells files:ijcep0036479.pdf|
|Scandinavian Journal of Immunology||Serum Proteomics Analysis in Rats of Immunosuppression Induced by Chronic Stress. pubmed:27384769|
|Saudi Journal of Gastroenterology||Deoxyschizandrin suppresses dss-induced ulcerative colitis in mice. pubmed:27976641|
|Biochemical and Biophysical Research Communications||MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis B pubmed:28342861|
|Virology||The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection pubmed:28988058|
|Antiviral Research||Coinjection of a vaccine and anti-viral agents can provide fast-acting protection from foot-and-mouth disease pubmed:28454913|
|Molecular Therapy. Methods & Clinical Development||Anti-high mobility group box 1 antibody ameliorates albuminuria in MRL/lprlupus-prone mice pubmed:28649578|
|Journal of Virology||Fucoidan from Fucus vesiculosus suppresses hepatitis B virus replication by enhancing extracellular signal-regulated Kinase activation. pubmed:28915824|
|Clinical Immunology||Toll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposis Pubmed:30056130|
|International forum of allergy & rhinology||Role of Toll‐like receptor 9 signaling on activation of nasal polyp–derived fibroblasts and its association with nasal polypogenesis Pubmed:29901280|
|Catalog No.||Related products for research use of Rhesus monkey (Simian) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA033Si01||Recombinant Interferon Alpha (IFNa)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA033Si01||Polyclonal Antibody to Interferon Alpha (IFNa)||WB; IHC; ICC; IP.|
|MAA033Si21||Monoclonal Antibody to Interferon Alpha (IFNa)||WB; IHC; ICC; IP.|
|SEA033Si||ELISA Kit for Interferon Alpha (IFNa)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|IEA033Si||Instant ELISA Kit for Interferon Alpha (IFNa)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA033Si||CLIA Kit for Interferon Alpha (IFNa)||Chemiluminescent immunoassay for Antigen Detection.|