ELISA Kit for Erythropoietin (EPO)
EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin
- Product No.SEA028Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range93.75-6,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 35.22pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Erythropoietin (EPO).
No significant cross-reactivity or interference between Erythropoietin (EPO) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Erythropoietin (EPO) and the recovery rates were calculated by comparing the measured value to the expected amount of Erythropoietin (EPO) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Erythropoietin (EPO) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Erythropoietin (EPO) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Erythropoietin (EPO) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Endocrinology||Prenatal Glucocorticoid Overexposure Causes Permanent Increases in Renal Erythropoietin Expression and Red Blood Cell Mass in the Rat Offspring Endo: source|
|Journal of Ethnopharmacology||Inhibitory effect of polysaccharides isolated from Angelica sinensis on hepcidin expression PubMed: 21333724|
|PLOS ONE||CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation PlosOne: Source|
|The Journal of Clinical Endocrinology & Metabolism||Alterations of Circulating Endothelial Cell and Endothelial Progenitor Cell Counts around the Ovulation PubMed: 22948762|
|Experimental & Molecular Medicine||Long-term and stable correction of uremic anemia by intramuscular injection of plasmids containing hypoxia-regulated system of erythropoietin expression PubMed: PMC3509184|
|Biochem Biophys Res Commun.||Reevaluation of erythropoietin production by the nephron Pubmed:24832733|
|Nutrition.||Carbohydrate and glutamine supplementation modulates the Th1/Th2 balance after exercise performed at a simulated altitude of 4500 m. Pubmed:2528040|
|J Appl Physiol (1985).||Decreased plasma soluble erythropoietin receptor in high-altitude excessive erythrocytosis and Chronic Mountain Sickness Pubmed:25324511|
|Expert Rev Proteomics||Plasma hepcidin in early-stage breast cancer patients: no relationship with interleukin-6, erythropoietin and erythroferrone PubMed: 26496240|
|Oncotarget||KIAA0101 is associated with human renal cell carcinoma proliferation and migration induced by erythropoietin pubmed:26575329|
|international journal of molecular sciences||Expression of Iron-Related Proteins Differentiate Non-Cancerous and Cancerous Breast Tumors. pubmed:28216608|
|Veterinary Clinical Pathology||Occult gastrointestinal bleeding is a common finding in dogs with chronic kidney disease pubmed:28186645|
|IOS Press Content Library||An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … articles:journal-of-alzheimers-disease|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|APA028Ra61||Active Erythropoietin (EPO)||Cell culture; Activity Assays.|
|EPA028Ra61||Eukaryotic Erythropoietin (EPO)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA028Ra01||Recombinant Erythropoietin (EPO)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA028Ra01||Polyclonal Antibody to Erythropoietin (EPO)||WB; IHC; ICC; IP.|
|MAA028Ra21||Monoclonal Antibody to Erythropoietin (EPO)||WB; IHC; ICC; IP.|
|SEA028Ra||ELISA Kit for Erythropoietin (EPO)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA028Ra||CLIA Kit for Erythropoietin (EPO)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA028Ra01||ELISA Kit DIY Materials for Erythropoietin (EPO)||Main materials for "Do It (ELISA Kit) Yourself".|