CLIA Kit for Interleukin 33 (IL33)

DV27; C9ORF26; IL1F11; NFHEV; Interleukin-1 Family, Member 11; Nuclear factor from high endothelial venules

Specificity

This assay has high sensitivity and excellent specificity for detection of Interleukin 33 (IL33).
No significant cross-reactivity or interference between Interleukin 33 (IL33) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 33 (IL33) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 33 (IL33) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-96 93
EDTA plasma(n=5) 93-101 97
heparin plasma(n=5) 86-94 89

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 33 (IL33) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 33 (IL33) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 33 (IL33) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-105% 82-101% 89-103% 85-93%
EDTA plasma(n=5) 97-104% 91-99% 83-96% 93-105%
heparin plasma(n=5) 88-104% 86-94% 97-105% 92-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

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Magazine Citations
The Korean Journal of Internal Medicine Interleukin-33, matrix metalloproteinase-9, and tissue ınhibitor of matrix metalloproteinase-1 in myocardial infarction PubMed: PMC3604606
Acta Histochem CXCL13 blockade attenuates lupus nephritis of MRL/lpr mice PubMed: 26456520
Journal of Interferon & Cytokine Research Epithelial Cell-Derived Cytokines Contribute to the Pathophysiology of Eosinophilic Chronic Rhinosinusitis Pubmed:26540312
Journal of Interferon & Cytokine Research The Expression and Regulation of Interleukin-33 in Human Epidermal Keratinocytes: A New Mediator of Atopic Dermatitis and Its Possible Signaling Pathway. pubmed:27348082
Archives of Dermatological Research IL-33 circulating serum levels are increased in patients with non-segmental generalized vitiligo pubmed:27388717
Sci Pharm. Chrysin Protects Rat Kidney from Paracetamol-Induced Oxidative Stress, Inflammation, Apoptosis, and Autophagy: A Multi-Biomarker Approach. pubmed:28134775
Biotechnology & Biotechnological Equipment  Association of IL-33, IL1RL1 gene polymorphisms with serum IL-33 levels and risk of asthma in adults and asthmatic bronchitis in children (Chinese) 10.1080:13102818.2018.1471361
Tropical Journal of Pharmaceutical Research Role of IL-33 and ST2 signaling and inflammatory responses in non-small cell lung cancer 172341
Particle and Fibre Toxicology Group II innate lymphoid cells and microvascular dysfunction from pulmonary titanium dioxide nanoparticle exposure Pubmed: 30413212
Scientific Reports Interleukin-33 serum levels in postmenopausal women with osteoporosis Pubmed: 30846811
Cell Reports Tumor-Derived Lactic Acid Contributes to the Paucity of Intratumoral ILC2s Pubmed: 32101749
Allergy Asthma & Immunology Research Relationship of microbial profile with airway immune response in eosinophilic or neutrophilic inflammation of asthmatics Pubmed: 32141256
Traditional Medicine Research Qingfei oral liquid downregulates TRPV1 expression to reduce airway inflammation and mucus hypersecretion injury caused by respiratory syncytial virus infection …
Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota
J Immunol Res Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus 34395633
Pathogens Elevated Levels of IL-33, IL-17 and IL-25 Indicate the Progression from Chronicity to Hepatocellular Carcinoma in Hepatitis C Virus Patients Pubmed:35056005
researchsquare Dexmedetomidine Attenuates Hyperalgesia Induced By Brachial Plexus Root Avulsion By Restoring The GLT-1 Function Via PKA Signaling
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