CLIA Kit for Myeloperoxidase (MPO)

Specificity

This assay has high sensitivity and excellent specificity for detection of Myeloperoxidase (MPO).
No significant cross-reactivity or interference between Myeloperoxidase (MPO) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Myeloperoxidase (MPO) and the recovery rates were calculated by comparing the measured value to the expected amount of Myeloperoxidase (MPO) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-90 86
EDTA plasma(n=5) 97-105 102
heparin plasma(n=5) 90-103 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myeloperoxidase (MPO) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myeloperoxidase (MPO) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Myeloperoxidase (MPO) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-96% 84-103% 98-105% 86-102%
EDTA plasma(n=5) 82-97% 96-105% 78-93% 80-95%
heparin plasma(n=5) 97-105% 98-105% 96-104% 79-94%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
International Journal of Medical Sciences Ozone Therapy and Hyperbaric Oxygen Treatment in Lung Injury in Septic Rats PubMed: 21234269
Respiration Protective Effect of Nicotine on Lipopolysaccharide-Induced Acute Lung Injury in Mice Karger: 000319151
Inflammation Preventive Effects of Valnemulin on Lipopolysaccharide-Induced Acute Lung Injury in Mice SpringerLink: 741437t67767777x
Advanced Materials Research A New Immunosensor for Serum Myeloperoxidase Based on Self-Assembly of Multi-Walled Carbon Nanotubes/Thionine/Gold Nanoparticles-Chitosan Scientific:1207
9 T cells that target carcinoembryonic antigen eradicate orthotopic pancreatic carcinomas without inducing autoimmune colitis in mice PubMed: 22750462
European Surgical Research Rosuvastatin Reloading before Cardiac Surgery with Cardiopulmonary Bypass PubMed: 23406839
Toxicology and Industrial Health Protective effects of Ankaferd blood stopper on aspirin-induced oxidative mucosal damage in a rat model of gastric injury. PubMed: 23114375
Experimental Lung Research Protective effect of pravastatin on lipopolysaccharide-induced acute lung injury during neutropenia recovery in mice Pubmed: 23368428
International Immunopharmacology Amentoflavone inhibits iNOS, COX-2 expression and modulates cytokine profile, NF-κB signal transduction pathways in rats with ulcerative colitis Pubmed: 24126114
Human & experimental toxicology Neuroprotective effects of α-ketoglutarate and ethyl pyruvate against motor dysfunction and oxidative changes caused by repeated 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine exposure in mice Pubmed: 23821591
International Immunopharmacology Protective effect of marine mangrove Rhizophora apiculata on acetic acid induced experimental colitis by regulating anti-oxidant enzymes, inflammatory mediators and nuclear factor-kappa B subunits ScienceDirect: S1567576913004153
PLOS ONE Novel CoQ10 Antidiabetic Mechanisms Underlie Its Positive Effect: Modulation of Insulin and Adiponectine Receptors, Tyrosine Kinase, PI3K, Glucose Transporters, sRAGE and Visfatin in Insulin Resistant/Diabetic Rats Pubmed:24586567
Acta Anaesthesiol Scand. Heparin-binding protein (HBP/CAP37) - a link to endothelin-1 in endotoxemia-induced pulmonary oedema? Pubmed:24611481
Journal of Environmental Pathology, Toxicology and Oncology Protective Effect of?Acacia ferruginea?against Ulcerative Colitis via Modulating Inflammatory Mediators, Cytokine Profile and NF-κB Signal Transduction Pathways Pubmed:24941292
European Journal of Pharmacology Neuroprotective effects of rosuvastatin against traumatic spinal cord injury in rats Pubmed:25084223
Iran J Pharm Res. Comparison of the Effects of Enoxaparin and Heparin on Inflammatory Biomarkers in Patients with ST-segment Elevated Myocardial Infarction: A prospective Open Label Pilot Clinical Trial Pubmed:25237354
Chem Biol Interact.? Gastroprotective effect of nymphayol isolated from Nymphaea stellata (Willd.) flowers: Contribution of antioxidant, anti-inflammatory and anti-apoptotic activities Pubmed:25289771
Journal of Surgical Research Melatonin attenuates intestinal ischemia–reperfusion-induced lung injury in rats by upregulating N-myc downstream-regulated gene 2 Pubmed:25491174
Neurochemistry International Sulfiredoxin-1 protects primary cultured astrocytes from ischemia-induced damage Pubmed:25620665
Ann N Y Acad Sci.? Modulating effect of Biophytum sensitivum extract on rats with acetic acid-induced ulcerative colitis Pubmed:5243883
J Crohns The Syk Inhibitor Fostamatinib Decreases the Severity of Colonic Mucosal Damage in a Rodent Model of Colitis PubMed: 26116555
Monoclon Antib Immunodiagn Immunother Preparation of Monoclonal Antibodies and a Simple Myeloperoxidase-Immunosorbent Assay for Detecting Human Myeloperoxidase Pubmed:26978734
Clinics and Research in Hepatology and Gastroenterology The efficacy of tyrosine kinase inhibitor dasatinib on colonic mucosal damage in murine model of colitis Pubmed:26823039
Asian J Pharm Clin Res THE ANTI-HYPERCHOLESTEROLEMIC EFFECT OF ULVAN POLYSACCHARIDE EXTRACTED FROM THE GREEN ALGA ULVAFASCIATA ON AGED HYPERCHOLESTEROLEMIC RATS journals:10835
Hepatology. Toll-like receptor 5 signaling restrains T-cell/natural killer T-cell activation and protects against concanavalin A-induced hepatic injury. pubmed:28273362
International Journal of Sports Physiology and Performance  Exploring the Potential of cfDNA Measurements After an Exhaustive Cycle Ergometer Test as a Marker for Performance Related Parameters pubmed:27617485
Journal of Pharmacy and Pharmaceutical Sciences Ellagic Acid Protects Against LPS-Induced Acute Lung Injury Through Inhibition of Nuclear Factor Kappa B, Proinflammatory Cytokines and Enhancement of Interleukin-10 c30adb97b06eb4f27063208df7acb7311368.pdf
Future Microbiology Transplantation of a bacterial consortium ameliorates trinitrobenzenesulfonic acid-induced colitisand intestinal dysbiosis in rats. pubmed:27415182
The Annals of Thoracic Surgery Sevoflurane Attenuates Ischemia-Reperfusion Injury in a Rat Lung Transplantation Model. pubmed:28190546
Biological Rhythm Research Fluctuation in the number, type and activity of blood neutrophils in cows exhibiting successful and unsuccessful completion of gestation cycle 09291016.2017.1311984
Journal of Cancer Research and Treatment Effect of Indole-3-carbinol and/or Metformin on Female Patients with Ulcerative Colitis (Premalignant Condition): Role of Oxidative Stress, Apoptosis and Proinflammatory Cytokines jcrt:5
Identification of Tengfu Jiangya Tablet Target Biomarkers journals:ecam
Hepatology. Hyperoxidized albumin modulates neutrophils to induce oxidative stress and inflammation in severe alcoholic hepatitis. pubmed:27775820
LAB ON A CHIP Enzyme catalysis-electrophoresis titration for multiplex enzymatic assay via moving reactionboundary chip. pubmed:27464600
Indian Journal of Animal Research Effect of Poly-herbal mixture and butyric acid feeding on immune parameters of postpartum Murrah buffaloes publication:299575009
The American Journal of Cardiology Frequency of Cholesterol Crystals in Culprit Coronary Artery Aspirate During Acute Myocardial Infarction and Their Relation to Inflammation and Myocardial Injury pubmed:28867129
Evidence-Based Complementary and Alternative Medicine Identification of Tengfu Jiangya Tablet Target Biomarkers with Quantitative Proteomic Technique pubmed:28408942
Lasers in Medical Science Effect of photobiomodulation therapy on reducingthe chemo-induced oral mucositis severity and on salivary levels of CXCL8/interleuki8, nitrite, and myeloperoxidase in patients undergoing hematopoietic stem cell transplantation: a randomized clinical trial article:10.1007
Experiental And Therapeutic Medicine Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescription pubmed:28672924
Frontiers in Immunology Upregulation of Intestinal Barrier Function in Mice with DSS-Induced Colitis by a Defined Bacterial Consortium Is Associated with Expansion of IL-17A Producing Gamma Delta T Cells 10.3389:fimmu.2017.00824
Journal of Pediatric Surgery Does penile tourniquet application alter bacterial adhesion to rat urethral cells: an in vitro study pubmed:28693848
Food and Agricultural Immunology Ellagic acid protects against LPS-induced acute lung injury through inhibition of nuclear factor kappa B, proinflammatory cytokines and enhancement of interleukin-10 10.1080/09540105.2017.1339670
Oxidative Medicine and Cellular Longevity Expression and Role of the Calcium-Sensing Receptor in Rat Peripheral Blood Polymorphonuclear Neutrophils pubmed:29081886
Advanced Science Letters Serum Myeloperoxidase in Stable Coronary Artery Disease 10.1166/asl.2017.8512
Tropical Journal of Pharmaceutical Research Hepatoprotective effect of taurine and coenzyme Q10 and their combination against acrylamide-induced oxidative stress in rats 10.4314/tjpr.v16i8.14
deposit once Role of NOD2 and S100A8/S100A9 in the pathogenesis of Coxsackievirus B3-induced myocarditis 10.14279/depositonce-5934
Archives of Medical Research Effects of Sulphasalazine in Cerebral Ischemia Reperfusion Injury in Rat. pubmed:28923326
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA601Hu01 Recombinant Myeloperoxidase (MPO) Positive Control; Immunogen; SDS-PAGE; WB.
PAA601Hu01 Polyclonal Antibody to Myeloperoxidase (MPO) WB; IHC; ICC; IP.
LAA601Hu71 Biotin-Linked Polyclonal Antibody to Myeloperoxidase (MPO) WB; IHC; ICC.
MAA601Hu22 Monoclonal Antibody to Myeloperoxidase (MPO) WB; IHC; ICC; IP.
SEA601Hu ELISA Kit for Myeloperoxidase (MPO) Enzyme-linked immunosorbent assay for Antigen Detection.
AEA601Hu ELISA Kit for Anti-Myeloperoxidase Antibody (Anti-MPO) Enzyme-linked immunosorbent assay for Antibody Detection.
SCA601Hu CLIA Kit for Myeloperoxidase (MPO) Chemiluminescent immunoassay for Antigen Detection.
KSA601Hu01 ELISA Kit DIY Materials for Myeloperoxidase (MPO) Main materials for "Do It (ELISA Kit) Yourself".