CLIA Kit for Thrombopoietin (TPO)

THPO; MGDF; MKCSF; ML; MPLLG; Myeloproliferative Leukemia Virus Oncogene Ligand; Megakaryocyte Growth And Development Factor; Megakaryocyte Stimulating Factor

Specificity

This assay has high sensitivity and excellent specificity for detection of Thrombopoietin (TPO).
No significant cross-reactivity or interference between Thrombopoietin (TPO) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Thrombopoietin (TPO) and the recovery rates were calculated by comparing the measured value to the expected amount of Thrombopoietin (TPO) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-105 102
EDTA plasma(n=5) 78-91 87
heparin plasma(n=5) 80-103 80

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thrombopoietin (TPO) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thrombopoietin (TPO) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Thrombopoietin (TPO) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-95% 91-99% 81-101% 80-103%
EDTA plasma(n=5) 87-103% 78-95% 79-101% 84-102%
heparin plasma(n=5) 78-91% 96-105% 78-89% 98-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
中国药理学通报 黄芪和当归的主要活性成分配伍对骨髓抑制小鼠造血功能的影响
Vaccine The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female rats Pubmed:35183388
Asian Journal of Transfusion Science A cross-sectional study on thrombopoietin levels in immune thrombocytopenia and its correlation with platelet count, megakaryocytes, and treatment response
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA135Hu01 Recombinant Thrombopoietin (TPO) Positive Control; Immunogen; SDS-PAGE; WB.
RPA135Hu03 Recombinant Thrombopoietin (TPO) Positive Control; Immunogen; SDS-PAGE; WB.
RPA135Hu04 Recombinant Thrombopoietin (TPO) Positive Control; Immunogen; SDS-PAGE; WB.
RPA135Hu02 Recombinant Thrombopoietin (TPO) Positive Control; Immunogen; SDS-PAGE; WB.
PAA135Hu01 Polyclonal Antibody to Thrombopoietin (TPO) WB; IHC; ICC; IP.
PAA135Hu02 Polyclonal Antibody to Thrombopoietin (TPO) WB; IHC; ICC; IP.
MAA135Hu22 Monoclonal Antibody to Thrombopoietin (TPO) WB; IHC; ICC; IP.
MEA135Hu Mini Samples ELISA Kit for Thrombopoietin (TPO) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA135Hu ELISA Kit for Thrombopoietin (TPO) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA135Hu CLIA Kit for Thrombopoietin (TPO) Chemiluminescent immunoassay for Antigen Detection.
LMA135Hu Multiplex Assay Kit for Thrombopoietin (TPO) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.