CLIA Kit for Bone Morphogenetic Protein 4 (BMP4)

BMP2B; BMP2-B; BMP2B1; ZYME; DVR4; Bone morphogenetic protein 2B

Specificity

This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 4 (BMP4).
No significant cross-reactivity or interference between Bone Morphogenetic Protein 4 (BMP4) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Bone Morphogenetic Protein 4 (BMP4) and the recovery rates were calculated by comparing the measured value to the expected amount of Bone Morphogenetic Protein 4 (BMP4) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-90 86
EDTA plasma(n=5) 80-105 102
heparin plasma(n=5) 78-90 82

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 4 (BMP4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 4 (BMP4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bone Morphogenetic Protein 4 (BMP4) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-101% 89-98% 80-97% 79-94%
EDTA plasma(n=5) 94-102% 82-89% 88-104% 91-101%
heparin plasma(n=5) 89-99% 98-105% 86-104% 80-90%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

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Magazine Citations
Journal of Cellular Biochemistry Titanium with Nanotopography Induces Osteoblast Differentiation by Regulating Endogenous Bone Morphogenetic Protein Expression and Signaling Pathway PubMed: 26681207
Domestic Animal Endocrinology Expression analysis of bone morphogenetic protein 4 between fat and lean birds in adipose tissue and serum Pubmed:26945137
FEBS Open Bio Bone morphogenetic protein 4 (BMP 4) regulates immortalized chicken preadipocyte proliferation by promoting G1/S cell cycle progression Pubmed: 30972973
Arch Gynecol Obstet The relationship between transforming growth factor β superfamily members (GDF11 and BMP4) and lumbar spine bone mineral density in postmenopausal Chinese … 34417839
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