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Recombinant Creatine Kinase, Muscle (CKM)

CK-MM; CKMM; CK3; CK-M; MM-CK; M-CK; Creatine kinase M-type, N-terminally processed

  • Product No.RPA109Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationCytoplasm
  • Predicted Molecular Mass41.5kDa
  • Accurate Molecular Mass41kDa(Analysis of differences refer to the manual)
  • Residues & TagsLys11~Leu367 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 97%
  • Isoelectric Point7.3
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 128  US$ 320  US$ 640  US$ 1920  US$ 4800 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Inflammation Assessment of endothelium and inflammatory response at the onset of reperfusion injury in hand surgery PubMed: 22583529
PLoS One Extraocular Muscle Characteristics Related to Myasthenia Gravis Susceptibility PubMed: PMC3568149
Kidney Int. Fructokinase activity mediates dehydration-induced renal injury Pubmed: 24336030
Methylxanthines and cardiotoxicity Methylxanthine induced cardiotoxicity and its mechanisms: An experimental study 230b:34bf146150b42971d76dda5e8329bc5f2f0b.pdf
Molecular Medicine Reports Protective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion Pubmed:29393473
Toxicological Sciences Proteomic and Metabolomic Profiling Identify Plasma Biomarkers for Exposure to Ultra-low Levels of Carfentanil Pubmed: 30321427
Clinical & Translational Oncology The effects of human sera conditioned by high-intensity exercise sessions and training on the tumorigenic potential of cancer cells Pubmed: 32447643
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