Packages (Simulation)

Polyclonal Antibody to Phosphofructokinase, Platelet (PFKP)

Image (I)

DAB staining on IHC-P; Samples: Human Lung cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human PFKP Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Cardiac Muscle Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Testis Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Cerebrum Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Spleen Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Cerebrum Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

Western Blot; Sample: Lane1: Porcine Heart lysate; Lane2: Porcine Skeletal muscle lysate; Lane3: A549 cell lysate; Lane4: Jurkat cell lysate Primary Ab: 0.3µg/ml Rabbit Anti-Human PFKP Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Selected
Western Blot; Sample: Lane1: A549 cell lysate; Lane2: Porcine Heart lysate; Lane3: Jurkat cell lysate; Lane4: Porcine Skeletal muscle lysate
Primary Ab: 3µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected
Knockout Varification:
Lane 1: Wild-type A549 cell lysate;
Lane 2: PFKP knockout A549 cell lysate;
Predicted MW: 85kd
Observed MW: 85kd
Primary Ab: 3µg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human PFKP Polyclonal Antibody (Catalog PAB745Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

Quality Guarantee

Instruction Manual

Polyclonal-Antibody-to-Phosphofructokinase--Platelet-(PFKP)-PAB745Hu01.pdf

Certificate

KO-Validated

Polyclonal Antibody to Phosphofructokinase, Platelet (PFKP)

PFK-C; PFKF; ATP-dependent 6-phosphofructokinase, platelet type; 6-phosphofructokinase type C; Phosphofructo-1-kinase isozyme C; Phosphohexokinase

Product No.PAB745Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB745Hu01-Recombinant Phosphofructokinase, Platelet (PFKP)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species MoreSus scrofa; Porcine (Pig)
ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 100 232 US$ 232 US$ 332 US$ 830 US$ 3320
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against PFKP. It has been selected for its ability to recognize PFKP in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
J Cell Mol Med	Huaier shows anti©\cancer activities by inhibition of cell growth, migration and energy metabolism in lung cancer through PI3K/AKT/HIF©\1¦Á pathway 33377619

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPB745Hu01	Recombinant Phosphofructokinase, Platelet (PFKP)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB745Hu01	Polyclonal Antibody to Phosphofructokinase, Platelet (PFKP)	WB,IHC
MAB745Hu22	Monoclonal Antibody to Phosphofructokinase, Platelet (PFKP)	WB; IHC; ICC; IP.
SEB745Hu	ELISA Kit for Phosphofructokinase, Platelet (PFKP)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB745Hu	Multiplex Assay Kit for Phosphofructokinase, Platelet (PFKP) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.