Packages (Simulation)
Image (I)
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DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human CNP Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human CNP Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Image (II)
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Figure. Western Blot; Sample: Recombinant CNP, Human.
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Western Blot; Sample: Human Lung lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human CNP Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Liver lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human CNP Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Liver lysate
Primary Ab: 0.2µg/ml Rabbit Anti-Human CNP Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human HepG2 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human CNP Polyclonal Antibody (Catalog PAA721Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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Instruction Manual
Certificate
Polyclonal Antibody to C-Type Natriuretic Peptide (CNP)
- Product No.PAA721Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA721Hu01-Recombinant C-Type Natriuretic Peptide (CNP)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species MoreMus musculus (Mouse)
- ApplicationsWB; IHC; FCM
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 95
US$ 221
US$ 316
US$ 790
US$ 3160
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against CNP. It has been selected for its ability to recognize CNP in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
For flow cytometry: 5-20µg/mL per 10^6 cells in 100 µl volume;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Human Reproduction | Pre-ovulatory LH/hCG surge decreases C-type natriuretic peptide secretion by ovarian granulosa cells to promote meiotic resumption of pre-ovulatory oocytes OxfordJournals: source |
| Sleep | Whole Genome DNA Methylation Analysis of Obstructive Sleep Apnea: IL1R2, NPR2, AR, SP140 Methylation and Clinical Phenotype. Pubmed:26888452 |
| Hormone Research in Paediatrics | Plasma C-Type Natriuretic Peptide: Emerging Applications in Disorders of Skeletal Growth Pubmed: 30844819 |
| European Journal of Nutrition | Tuscany Sangiovese grape juice imparts cardioprotection by regulating gene expression of cardioprotective C-type natriuretic peptide Pubmed: 31707544 |
| Peptides | Osteocrin alleviates cardiac hypertrophy via attenuating oxidative stress Pubmed:35218795 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA721Hu01 | Recombinant C-Type Natriuretic Peptide (CNP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA721Hu21 | OVA Conjugated C-Type Natriuretic Peptide (CNP) | Immunogen; SDS-PAGE; WB. |
| PAA721Hu01 | Polyclonal Antibody to C-Type Natriuretic Peptide (CNP) | WB; IHC; FCM |
| PAA721Hu08 | Polyclonal Antibody to C-Type Natriuretic Peptide (CNP) | WB; IHC; ICC; IP. |
| MAA721Hu22 | Monoclonal Antibody to C-Type Natriuretic Peptide (CNP) | WB; IHC; ICC; IP. |
| SEA721Hu | ELISA Kit for C-Type Natriuretic Peptide (CNP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA721Hu | ELISA Kit for C-Type Natriuretic Peptide (CNP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA721Hu | Multiplex Assay Kit for C-Type Natriuretic Peptide (CNP) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA721Hu01 | ELISA Kit DIY Materials for C-Type Natriuretic Peptide (CNP) | Main materials for "Do It (ELISA Kit) Yourself". |