Packages (Simulation)

Image (I)

IHC Images

Image (II)

Figure. Western Blot; Sample: Recombinant NES, Mouse.
Mouse BALB/c 3T3 cells were fixed with 1% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-mouse NES Polyclonal Antibody (Catalog PAA500Mu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

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Instruction Manual

Polyclonal-Antibody-to-Nestin-(NES)-PAA500Mu01.pdf

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Polyclonal Antibody to Nestin (NES)

Product No.PAA500Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA500Mu01-Recombinant Nestin (NES)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species Moren/a
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 97 227 US$ 227 US$ 324 US$ 810 US$ 3240
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against NES. It has been selected for its ability to recognize NES in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Kidney and Blood Pressure Research	Nestin Improves Preeclampsia-Like Symptoms by Inhibiting Activity of Cyclin-Dependent Kinase 5 Pubmed:29689548
Neural Plast	P2Y2 Receptor Mediated Neuronal Regeneration and Angiogenesis to Affect Functional Recovery in Rats with Spinal Cord Injury Pubmed:35154311

Catalog No.	Related products for research use of Mus musculus (Mouse) Organism species	Applications (RESEARCH USE ONLY!)
RPA500Mu02	Recombinant Nestin (NES)	Positive Control; Immunogen; SDS-PAGE; WB.
RPA500Mu01	Recombinant Nestin (NES)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA500Mu02	Polyclonal Antibody to Nestin (NES)	WB; IHC; ICC; IP.
PAA500Mu01	Polyclonal Antibody to Nestin (NES)	WB; IHC; ICC; IP.
SEA500Mu	ELISA Kit for Nestin (NES)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA500Mu	Multiplex Assay Kit for Nestin (NES) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.