Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2)

COL1-A2; OI4; Collagen Alpha-2(I)chain; osteogenesis imperfecta type IV; Collagen Of Skin,Tendon And bone,Alpha-2 Chain

  • Product No.PAA215Bo01
  • Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
  • SourcePolyclonal antibody preparation
  • HostRabbit
  • Potencyn/a
  • Ig Type IgG
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration500µg/mL
  • Organism Species Moren/a
  • ApplicationsWB; IHC; ICC; IP.
    If the antibody is used in flow cytometry, please check FCM antibodies.
  • Downloadn/a
  • UOM 20µl100µl 200µl 1ml 10ml
  • FOB US$ 113  US$ 265  US$ 378  US$ 945  US$ 3780 
    For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against COL1a2. It has been selected for its ability to recognize COL1a2 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Plos one Inhibition of TGF-β Signaling Enables Human Corneal Endothelial Cell Expansion In Vitro for Use in Regenerative Medicine PubMed: PMC3581499
J Proteomics Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC) PubMed: 26718731
PLoS One Pro-fibrotic compounds induce stellate cell activation, ECM-remodelling and Nrf2 activation in a human 3D-multicellular model of liver fibrosis pubmed:28665955
American Journal of Translational Research A novel hybrid 3D-printed titanium scaffold for osteogenesis in a rabbit calvarial defect model Pubmed:29511441
Sci Rep The effect of hypoxia on the proteomic signature of pig adipose-derived stromal/stem cells (pASCs) Pubmed: 33208768
Experimental and Therapeutic Medicine Protective effects of halophyte complex extract against UVB?induced damage in human keratinocytes and the skin of hairless mice 33986847
Catalog No. Related products for research use of Bos taurus; Bovine (Cattle) Organism species Applications (RESEARCH USE ONLY!)
RPA215Bo01 Recombinant Collagen Type I Alpha 2 (COL1a2) Positive Control; Immunogen; SDS-PAGE; WB.
PAA215Bo01 Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2) WB; IHC; ICC; IP.
MAA215Bo21 Monoclonal Antibody to Collagen Type I Alpha 2 (COL1a2) WB; IHC; ICC; IP.
SEA215Bo ELISA Kit for Collagen Type I Alpha 2 (COL1a2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA215Bo Multiplex Assay Kit for Collagen Type I Alpha 2 (COL1a2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.