Mini Samples ELISA Kit for Angiopoietin Like Protein 8 (ANGPTL8)
RIFL; TD26; C19orf80; Lipasin; Betatrophin; Hepatocellular Carcinoma-Associated Protein TD26
- Product No.MEW803Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.059ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Mini Samples Angiopoietin Like Protein 8 (ANGPTL8).
No significant cross-reactivity or interference between Mini Samples Angiopoietin Like Protein 8 (ANGPTL8) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Mini Samples Angiopoietin Like Protein 8 (ANGPTL8) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Angiopoietin Like Protein 8 (ANGPTL8) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Angiopoietin Like Protein 8 (ANGPTL8) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Angiopoietin Like Protein 8 (ANGPTL8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Angiopoietin Like Protein 8 (ANGPTL8) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×60µL||Assay Diluent A||1×6mL|
|Detection Reagent B||1×60µL||Assay Diluent B||1×6mL|
|TMB Substrate||1×4.5mL||Stop Solution||1×3mL|
|Wash Buffer (30 × concentrate)||1×10mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.
|PLoS One||Increased Maternal and Cord Blood Betatrophin in Gestational Diabetes PubMed: 26115519|
|Nutrición Hospitalaria||Serum betatrophin level increased in subjects with metabolic syndrome: a case-control study wp-content:10563|
|Mediators of Inflammation||Serum betatrophin level increased in subjects with nonalcoholic fatty liver disease files:ijcem0020951.pdf|
|PLoS One||Resolving Discrepant Findings on ANGPTL8 in β-Cell Proliferation: A Collaborative Approach to Resolving the Betatrophin Controversy pubmed:27410263|
|Obesity Research and Clinical Practice||Relationship between serum betatrophin levels and the first-phase of glucose-stimulated insulin secretion pubmed:27554132|
|打不开网页||Aumento del nivel de betatrofina en suero en sujetos con síndrome metabólico: estudio de casos y controles :|
|DIABETES & METABOLISM||Levels of betatrophin decrease during pregnancy despite increased insulin resistance, beta-cellfunction and triglyceride levels. pubmed:27555469|
|Decreased circulating levels of betatrophin in Chinese women with polycystic ovary syndrome :|
|International Journal of Endocrinology||Serum concentrations of betatrophin and its association with indirect indices of insulin resistance and beta cell function in women with polycystic ovary … pubmed:28702052|
|Diabetes Research and Clinical Practice||Angiopoietin-like protein 8: an attractive biomarker for the evaluation of subjects with insulin resistance and related disorders pubmed:28965028|
|Diabetes/Metabolism Research and Reviews||ANGPTL8 (Betatrophin) Role in Diabetes and Metabolic Diseases pubmed:28722798|
|Bibliography||The Correlation Between Circulating Betatrophin and Insulin Resistance in General Population: A Meta-Analysis pubmed:28931172|
|Frontiers in Endocrinology||ANGPTL8: an important regulator in metabolic disorders Pubmed:29719529|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPW803Hu01||Recombinant Angiopoietin Like Protein 8 (ANGPTL8)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAW803Hu01||Polyclonal Antibody to Angiopoietin Like Protein 8 (ANGPTL8)||WB; IHC; ICC; IP.|
|MAW803Hu21||Monoclonal Antibody to Angiopoietin Like Protein 8 (ANGPTL8)||WB; IHC; ICC; IP.|
|SEW803Hu||ELISA Kit for Angiopoietin Like Protein 8 (ANGPTL8)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|MEW803Hu||Mini Samples ELISA Kit for Angiopoietin Like Protein 8 (ANGPTL8)||Enzyme-linked immunosorbent assay for Antigen Detection.|