Mini Samples ELISA Kit for Interleukin 22 (IL22)

ILTIF; Zcyto18; IL-TIF; IL-D110; TIFa; TIFIL-23; IL-22; Cytokine Zcyto18; IL-10-Related T-Cell-Derived Inducible Factor

Specificity

This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 22 (IL22).
No significant cross-reactivity or interference between Mini Samples Interleukin 22 (IL22) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Interleukin 22 (IL22) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Interleukin 22 (IL22) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 93-101 97
EDTA plasma(n=5) 90-99 94
heparin plasma(n=5) 94-103 99

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 22 (IL22) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 22 (IL22) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Interleukin 22 (IL22) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-102% 95-104% 79-95% 82-89%
EDTA plasma(n=5) 90-101% 80-103% 92-101% 94-102%
heparin plasma(n=5) 90-102% 86-104% 90-98% 85-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×4.5mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Biology of Blood and Marrow Transplatation Reciprocal Expression of Enteric Antimicrobial Proteins in Intestinal Graft-Versus-Host Disease Pubmed: 23927965
Inflammation Protein Kinase C Theta Inhibition Attenuates Lipopolysaccharide-Induced Acute Lung Injury through Notch Signaling Pathway via Suppressing Th17 Cell Response … Pubmed: 31297750
Free Radical Biology and Medicine Vitamin D protects against oxidative stress, inflammation and hepatorenal damage induced by acute paracetamol toxicity in rat Pubmed: 31255668
Microbial Pathogenesis Tangeretin attenuates lipopolysaccharide-induced acute lung injury through Notch signaling pathway via suppressing Th17 cell response in mice Pubmed: 31676364
Journal of Cosmetic Dermatology Serum Level of Interleukin 22 in Patients with Cutaneous Warts: A Case Control Study Pubmed: 33043549
Biomed Research International Effects of 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis Pubmed: 32908870
JOURNAL OF MICROBIOLOGY Melatonin attenuates microbiota dysbiosis of jejunum in short-term sleep deprived mice Pubmed: 32424577
J Periodontol Cytokine profile in serum and gingival crevicular fluid of children with inflammatory bowel disease: A case‐control study 34730850
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPC032Mu01 Recombinant Interleukin 22 (IL22) Positive Control; Immunogen; SDS-PAGE; WB.
RPC032Mu02 Recombinant Interleukin 22 (IL22) Positive Control; Immunogen; SDS-PAGE; WB.
APC032Mu61 Active Interleukin 22 (IL22) Cell culture; Activity Assays.
PAC032Mu02 Polyclonal Antibody to Interleukin 22 (IL22) WB; IHC; ICC; IP.
PAC032Mu01 Polyclonal Antibody to Interleukin 22 (IL22) WB; IHC; ICC; IP.
SEC032Mu ELISA Kit for Interleukin 22 (IL22) Enzyme-linked immunosorbent assay for Antigen Detection.
MEC032Mu Mini Samples ELISA Kit for Interleukin 22 (IL22) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC032Mu CLIA Kit for Interleukin 22 (IL22) Chemiluminescent immunoassay for Antigen Detection.
LMC032Mu Multiplex Assay Kit for Interleukin 22 (IL22) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.