Mini Samples ELISA Kit for Prion Protein (PRNP)

CD230; GSS; ASCR; CJD; PRIP; PrP; PrP2730; PrPc; Major prion protein; Creutzfeldt-Jakob Disease; Gerstmann-Strausler-Scheinker Syndrome; Fatal Familial Insomnia

Specificity

This assay has high sensitivity and excellent specificity for detection of Mini Samples Prion Protein (PRNP).
No significant cross-reactivity or interference between Mini Samples Prion Protein (PRNP) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Prion Protein (PRNP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Prion Protein (PRNP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×4.5mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
FASEB Journal Altered levels of plasma neuron-derived exosomes and their cargo proteins characterize acute and chronic mild traumatic brain injury Pubmed: 30605353
Journal of Integrative Neuroscience Effects of cellular prion protein on rapid eye movement sleep deprivation-induced spatial memory impairment Pubmed: 31912703
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
EPB680Hu61 Eukaryotic Prion Protein (PRNP) Positive Control; Immunogen; SDS-PAGE; WB.
RPB680Hu01 Recombinant Prion Protein (PRNP) Positive Control; Immunogen; SDS-PAGE; WB.
PAB680Hu01 Polyclonal Antibody to Prion Protein (PRNP) WB; IHC; ICC; IP.
PAB680Hu02 Polyclonal Antibody to Prion Protein (PRNP) WB; IHC; ICC; IP.
MAB680Hu21 Monoclonal Antibody to Prion Protein (PRNP) WB; IHC; ICC; IP.
HEB680Hu High Sensitive ELISA Kit for Prion Protein (PRNP) Enzyme-linked immunosorbent assay for Antigen Detection.
MEB680Hu Mini Samples ELISA Kit for Prion Protein (PRNP) Enzyme-linked immunosorbent assay for Antigen Detection.
SEB680Hu ELISA Kit for Prion Protein (PRNP) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB680Hu Multiplex Assay Kit for Prion Protein (PRNP) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB680Hu01 ELISA Kit DIY Materials for Prion Protein (PRNP) Main materials for "Do It (ELISA Kit) Yourself".