Mini Samples ELISA Kit for Interleukin 27A (IL27A)

p28; IL30; Interleukin 30; Interleukin-27 subunit alpha; IL-27 subunit alpha; IL27-A

Specificity

This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 27A (IL27A).
No significant cross-reactivity or interference between Mini Samples Interleukin 27A (IL27A) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Interleukin 27A (IL27A) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Interleukin 27A (IL27A) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-99 95
EDTA plasma(n=5) 79-89 79
heparin plasma(n=5) 94-102 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 27A (IL27A) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 27A (IL27A) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Interleukin 27A (IL27A) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-97% 97-105% 79-104% 88-101%
EDTA plasma(n=5) 81-94% 89-103% 79-101% 89-97%
heparin plasma(n=5) 88-104% 78-101% 91-102% 82-90%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×4.5mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Human Immunology Elevated interleukin-27 enhances the polarization of Th1/Tc1 cells and the production of proinflammatory cytokines in primary immune thrombocytopenia Pubmed: 22210084
Front Pharmacol Poria Attenuates Idiosyncratic Liver Injury Induced by Polygoni Multiflori Radix Praeparata pubmed:27803670
cyberleninka КЛИНИКО-ЭПИДЕМИОЛОГИЧЕСКАЯ ХАРАКТЕРИСТИКА И ОСОБЕННОСТИ БАЛЛАНСА ЦИТОКИНОВ ПРИ КЛЕЩЕВОМ ЭНЦЕФАЛИТЕ В …
Int J Lab Hematol Interlukin‐27 rs153109 polymorphism confers the susceptibility and prognosis of aplastic anemia in Chinese population 34528397
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
EPA385Mu61 Eukaryotic Interleukin 27A (IL27A) Positive Control; Immunogen; SDS-PAGE; WB.
APA385Mu61 Active Interleukin 27A (IL27A) Cell culture; Activity Assays.
RPA385Mu01 Recombinant Interleukin 27A (IL27A) Positive Control; Immunogen; SDS-PAGE; WB.
PAA385Mu01 Polyclonal Antibody to Interleukin 27A (IL27A) WB; IHC; ICC; IP.
SEA385Mu ELISA Kit for Interleukin 27A (IL27A) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA385Mu Mini Samples ELISA Kit for Interleukin 27A (IL27A) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA385Mu Multiplex Assay Kit for Interleukin 27A (IL27A) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.