Packages (Simulation)
Image (I)
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DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 30µg/ml Mouse Anti-Human HPD Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 30µg/ml Mouse Anti-Human HPD Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 30µg/ml Mouse Anti-Human HPD Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 30µg/ml Mouse Anti-Human HPD Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
FITC staining on IF;
Samples: Human HepG2 cell;
Primary Ab: 20μg/ml Mouse Anti-Human HPD Antibody
Second Ab: 1μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18) -
FITC staining on IF;
Sample: HepG2 cell
Primary Ab: 20µg/ml Mouse Anti-Human HPD Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
Image (II)
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Western Blot Sample: Lane1: Human Liver lysate; Lane2: Porcine Kidney lysate; Lane3: HepG2 cell lysate;
Primary Ab: 2µg/ml Mouse Anti-Human HPD Antibody
Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Quality Guarantee
Instruction Manual
Certificate
Monoclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD)
PPD; 4-HPPD; 4HPPD; GLOD3; Glyoxalase Domain Containing 3; 4-hydroxyphenylpyruvic acid oxidase
- Product No.MAE251Hu21
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)D2
- Ig Isotype IgG2a Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPE251Hu01-Recombinant 4-Hydroxyphenylpyruvate Dioxygenase (HPD)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration1mg/ml
- Organism Species MoreSus scrofa; Porcine (Pig)
- ApplicationsWB; IHC; ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 111
US$ 259
US$ 370
US$ 925
US$ 3700
For more details, please contact local distributors!
SPECIFITY
The antibody is a mouse monoclonal antibody raised against HPD. It has been selected for its ability to recognize HPD in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-30µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| Exp Gerontol. | Age associated decline in the conversion of leucine to β-Hydroxy-β-Methylbutyrate in rats Pubmed:27073170 |