Packages (Simulation)

Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2)

Image (I)

DAB staining on IHC-P; Sample: Human Ovarian cancer Tissue; Primary Ab: 10ug/ml Mouse Anti-Multi-species ACTa2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Rectum Tissue;
Primary Ab: 10ug/ml Mouse Anti-Multi-species ACTa2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Rat Skeletal muscle Tissue;
Primary Ab: 3ug/ml Mouse Anti-Multi-species ACTa2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Rat Lung Tissue;
Primary Ab: 3ug/ml Mouse Anti-Multi-species ACTa2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Mouse Pancreas Tissue;
Primary Ab: 3ug/ml Mouse Anti-Multi-species ACTa2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Mouse Spleen Tissue;
Primary Ab: 3ug/ml Mouse Anti-Multi-species ACTa2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

Image (II)

Western Blot; Sample: Lane1: Human Placenta lysate; Lane2: Porcine Heart lysate; Lane3: Mouse Heart lysate; Lane4: Mouse Skeletal muscle lysate Primary Ab: 0.01μg/ml Mouse Anti-Multi-species ACTa2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19
Western Blot; Sample: Lane1: Rat Heart lysate; Lane2: Canine Heart lysate
Primary Ab: 0.001µg/ml Mouse Anti-Multi-species ACTa2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

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Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2)

ACT-A2; ACTSA; ACTVS; Asma; GIG46; Cell growth-inhibiting gene 46 protein

Product No.MAB342Mi21
Organism SpeciesMus musculus (Mouse), Rattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourceMonoclonal antibody preparation
HostMouse
Potency (Clone Number)n/a
Ig Isotype IgG
PurificationProtein A + Protein G affinity chromatography
LabelNone
Immunogen n/a
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration1mg/mL
Organism Species MoreHomo sapiens (Human), Mus musculus (Mouse), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Downloadn/a
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 116
For more details, please contact local distributors! US$ 270
For more details, please contact local distributors! US$ 386
For more details, please contact local distributors! US$ 965
For more details, please contact local distributors! US$ 3860
For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against ACTa2. It has been selected for its ability to recognize ACTa2 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Cell Mol Immunol	Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica Pubmed:27157496
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY	Effects of repeated increasing doses of cisplatin as models of acute kidney injury and chronic kidney disease in rats Pubmed: 32936352
Brazilian Journal of Pharmaceutical Sciences	Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm

Catalog No.	Related products for research use of Mus musculus (Mouse), Rattus norvegicus (Rat) Organism species	Applications (RESEARCH USE ONLY!)
RPB342Mi01	Recombinant Actin Alpha 2, Smooth Muscle (ACTa2)	Positive Control; Immunogen; SDS-PAGE; WB.
CAB342Mi01	Anti-Actin Alpha 2, Smooth Muscle (ACTa2) Polyclonal Antibody	Loading Control of WB.
PAB342Mi01	Polyclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2)	WB; IHC; ICC; IP.
MAB342Mi21	Monoclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2)	WB; IHC; ICC; IP.
SEB342Mi	ELISA Kit for Actin Alpha 2, Smooth Muscle (ACTa2)	Enzyme-linked immunosorbent assay for Antigen Detection.