Packages (Simulation)

Image (I)

DAB staining on IHC-P; Samples: Human Stomach Tissue; Primary Ab: 20µg/ml Mouse Anti-Human NES Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue

Image (II)

Figure. Western Blot; Sample: Recombinant NES, Human.
Figure. Western Blot; Sample: Rat Heart Tissue.

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Instruction Manual

Monoclonal-Antibody-to-Nestin-(NES)-MAA500Hu22.pdf

Certificate

Monoclonal Antibody to Nestin (NES)

Product No.MAA500Hu22
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourceMonoclonal antibody preparation
HostMouse
Potency (Clone Number)D4
Ig Isotype IgG2b Kappa
PurificationProtein A + Protein G affinity chromatography
LabelNone
Immunogen RPA500Hu01-Recombinant Nestin (NES)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration0.75mg/ml
Organism Species MoreRattus norvegicus (Rat)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 101 235 US$ 235 US$ 336 US$ 840 US$ 3360
For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against NES. It has been selected for its ability to recognize NES in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Kidney and Blood Pressure Research	Nestin Improves Preeclampsia-Like Symptoms by Inhibiting Activity of Cyclin-Dependent Kinase 5 Pubmed:29689548
Neural Plast	P2Y2 Receptor Mediated Neuronal Regeneration and Angiogenesis to Affect Functional Recovery in Rats with Spinal Cord Injury Pubmed:35154311

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPA500Hu01	Recombinant Nestin (NES)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA500Hu01	Polyclonal Antibody to Nestin (NES)	WB; IHC; ICC; IP.
LAA500Hu51	APC-Linked Polyclonal Antibody to Nestin (NES)	WB; IHC; ICC; IF.
MAA500Hu27	Monoclonal Antibody to Nestin (NES)	WB; IHC; ICC; IP.
MAA500Hu21	Monoclonal Antibody to Nestin (NES)	WB; IHC; ICC; IP.
MAA500Hu23	Monoclonal Antibody to Nestin (NES)	WB; IHC; ICC; IP.
MAA500Hu24	Monoclonal Antibody to Nestin (NES)	WB; IHC; ICC; IP.
MAA500Hu25	Monoclonal Antibody to Nestin (NES)	WB; IHC; ICC; IP.
MAA500Hu22	Monoclonal Antibody to Nestin (NES)	WB; IHC; ICC; IP.
FAA500Hu08	Anti-Nestin (NES) Monoclonal Antibody	Flow cytometry.
FAA500Hu04	Anti-Nestin (NES) Monoclonal Antibody	Flow cytometry.
FAA500Hu06	Anti-Nestin (NES) Monoclonal Antibody	Flow cytometry.
SEA500Hu	ELISA Kit for Nestin (NES)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA500Hu	Multiplex Assay Kit for Nestin (NES) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.