Multiplex Assay Kit for Lactate Dehydrogenase (LDH) ,etc. by FLIA (Flow Luminescence Immunoassay)

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Lactate Dehydrogenase (LDH) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Lactate Dehydrogenase (LDH) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Lactate Dehydrogenase (LDH) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Lactate Dehydrogenase (LDH) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-97 94
EDTA plasma(n=5) 89-96 92
heparin plasma(n=5) 93-104 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactate Dehydrogenase (LDH) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactate Dehydrogenase (LDH) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lactate Dehydrogenase (LDH) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-105% 84-104% 89-97% 81-101%
EDTA plasma(n=5) 81-97% 83-97% 81-91% 90-97%
heparin plasma(n=5) 87-102% 80-95% 81-92% 80-94%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:LDH) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Neuroscience Research Hydrogen sulfide antagonizes homocysteine-induced neurotoxicity in PC12 cells PubMed: 20674619
Clinical and Experimental Pharmacology and Physiology Formaldehyde induces neurotoxicity to PC12 cells involving inhibition of paraoxonase-1 expression and activity PubMed: 21261675
Neurochemistry International Hydrogen sulfide prevents formaldehyde-induced neurotoxicity to PC12 cells by attenuation of mitochondrial dysfunction and pro-apoptotic potential ScienceDirect: S0197018612001428
ostaglandins Leukot Essent Fatty Acids. Docosahexaenoic and eicosapentaenoic acid supplementation does not exacerbate oxidative stress or intravascular haemolysis in homozygous sickle cell patients. Pubmed: 24095588
Journal of Diabetes and its Complications Nerve growth factor rescues diabetic mice heart after ischemia/reperfusion injury via up-regulation of the TRPV1 receptor Pubmed:25650182
Cancer Science & Therapy An Additional risk of Lung Cancer from Recurrent Exposure to Ethyl Carbamate (EC) in BALB/C Mice Open-Access: 1000375
Journal of Antimicrobial Chemotherapy NLF20: an antimicrobial peptide with therapeutic potential against invasive Pseudomonas aeruginosa infection PubMed: 26503666
American Journal of Physology Heart and Circulatory Physiology Dysfunction of mitochondria and deformed gap junctions in the heart of IL-18-deficient mice Pubmed:27288439
Translational Research Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodules Pubmed:27172385
Experimental Cell Research UpreCavia (Guinea pig )lation of CBS/H2S system contributes to asymmetric dimethylarginine-triggered protection against the neurotoxicity of glutamate to PC12 cells by inhibiting NOS/NO pathway science:S0014482716301598
Blood Cells Mol Dis Transgenic sickle cell trait mice do not exhibit abnormal thermoreCavia (Guinea pig )latory and stress responses to heat shock exposure Pubmed:27282581
PLoS One. Outer Balloon Ligation Increases Success Rate of Ischemia-Reperfusion Injury Model in Mice. pubmed:27907155
Scientific Reports Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway. pubmed:27678302
Experimental Biology and Medicine Ameliorating effect of anti-Alzheimer’s drugs on the bidirectional association between type 2 diabetes mellitus and Alzheimer’s disease pubmed:28534431
Cancer Science A Novel Potential Strategy for Enhancing Antigen Presentation of Human Hepatocellular Carcinoma Cells Propagated ex-vivo Using a Ribonuclease Enzyme … Scholarena:Source
Molecular Medicine Reports Protective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion Pubmed:29393473
Molecular Medicine Reports  Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats Pubmed:29956781
Oncology Letters A novel potential effective strategy for enhancing the antitumor immune response in breast cancer patients using a viable cancer cell‑dendritic cell‑based vaccine Pubmed:29928442
Experimental Allergy and Immunology Osteopontin protects against pneumococcal infection in a murine model of allergic airway inflammation Pubmed: 30362569
Scientific Reports Low plasma haptoglobin is a risk factor for life-threatening childhood severe malarial anemia and not an exclusive consequence of hemolysis Pubmed: 30510258
Molecular Medicine Reports Differentially expressed lncRNAs, miRNAs and mRNAs with associated ceRNA networks in a mouse model of myocardial ischemia/reperfusion injury Pubmed: 32705277
Cell Transplant Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stromal Cells Protect Cardiac Cells Against Hypoxia/Reoxygenation Injury by Inhibiting … Pubmed: 32864999
Nanomaterials Biological Safety and Biodistribution of Chitosan Nanoparticles Pubmed: 32340313
Journal of Renal Injury Prevention Prevention of kidney injury after myocardial ischemia reperfusion is achievable with short-term protein restriction
clinical and translational medicine Milk fat globule EGF factor 8 restores mitochondrial function via integrin©\medicated activation of the FAK©\STAT3 signaling pathway in acute pancreatitis 33634976
Biomed Res Int Factors Affecting the Incidence, Progression, and Severity of COVID-19 in Type 1 Diabetes Mellitus 34840966
Cell Cycle Improvement of autophagic flux mediates the protection of hydrogen sulfide against arecoline-elicited neurotoxicity in PC12 cells Pubmed:35316162
Biomed Res Int A Famous Chinese Medicine Formula: Yinhuo Decoction Antagonizes the Damage of Corticosterone to PC12 Cells and Improves Depression by Regulating … Pubmed:35281603
Int Immunopharmacol LncRNA KCNQ1OT1 attenuates myocardial injury induced by hip fracture via regulating of miR-224-3p/GATA4 axis Pubmed:35217336
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
PAB864Ra01 Polyclonal Antibody to Lactate Dehydrogenase (LDH) WB; IHC; ICC; IP.
MAB864Ra21 Monoclonal Antibody to Lactate Dehydrogenase (LDH) WB; IHC; ICC; IP.
SEB864Ra ELISA Kit for Lactate Dehydrogenase (LDH) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB864Ra Multiplex Assay Kit for Lactate Dehydrogenase (LDH) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.