ELISA Kit DIY Materials for Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c)

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c). No significant cross-reactivity or interference between Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Pediatric Diabetes  Circulating MOTS‐c levels are decreased in obese male children and adolescents and associated with insulin resistance Pubmed:29691953
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