ELISA Kit DIY Materials for Hypoxia Inducible Factor 2 Alpha (HIF2a)

EPAS1; HLF; MOP2; PASD2; BHLHE73; Endothelial PAS Domain 1; HIF-1 Alpha-Like Factor; Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73

  • Product No.KSD466Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range78-5000pg/mL
  • Applicable Sensitivity29pg/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1368  US$ 2280  US$ 4104  US$ 7980  US$ 13680 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Hypoxia Inducible Factor 2 Alpha (HIF2a). No significant cross-reactivity or interference between Hypoxia Inducible Factor 2 Alpha (HIF2a) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Neuro Oncol Combination viroimmunotherapy with checkpoint inhibition to treat glioma, based on location-specific tumor profiling PubMed: 26409567
Clinical Trials in Degenerative Diseases Correlating oxidative stress-related factors with bone metabolic markers in older adult male patients exhibiting degenerative osteoporosis in the high-altitude hypoxic area of China: study protocol for a non-randomized controlled trial issn:2542-3975
Journal of Physiological Anthropology Telomere elongation protects heart and lung tissue cells from fatal damage in rats exposed to severe hypoxia Pubmed:29454386
Oxidative damage and iron dyshomeostasis in thalassemic patients
Neuropsychiatric Disease and Treatment The Association Between Hypoxia Improvement and Electroconvulsive Therapy for Major Depressive Disorder 34588778
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPD466Ra01 Recombinant Hypoxia Inducible Factor 2 Alpha (HIF2a) Positive Control; Immunogen; SDS-PAGE; WB.
PAD466Ra01 Polyclonal Antibody to Hypoxia Inducible Factor 2 Alpha (HIF2a) WB; IHC; ICC; IP.
LAD466Ra71 Biotin-Linked Polyclonal Antibody to Hypoxia Inducible Factor 2 Alpha (HIF2a) WB; IHC; ICC.
MAD466Ra21 Monoclonal Antibody to Hypoxia Inducible Factor 2 Alpha (HIF2a) WB; IHC; ICC; IP.
SED466Ra ELISA Kit for Hypoxia Inducible Factor 2 Alpha (HIF2a) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD466Ra Multiplex Assay Kit for Hypoxia Inducible Factor 2 Alpha (HIF2a) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSD466Ra01 ELISA Kit DIY Materials for Hypoxia Inducible Factor 2 Alpha (HIF2a) Main materials for "Do It(ELISA Kit) Yourself"