ELISA Kit DIY Materials for Cathelicidin Antimicrobial Peptide (CAMP)

CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Cathelicidin Antimicrobial Peptide (CAMP). No significant cross-reactivity or interference between Cathelicidin Antimicrobial Peptide (CAMP) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
University of Waikato The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis Waikato:Source
Clin Infect Dis. Host immune response to tuberculous meningitis Pubmed:25301213
Vaccine On birth single dose live attenuated OPV and BCG vaccination induces gut cathelicidin LL37 responses at 6 week of age: A natural experiment Pubmed:25444792
Urology Modulating the Internalization of Bacille Calmette-Guérin by Cathelicidin in Bladder Cancer Cells Pubmed:25681250
Urology Modulating the Internalization of Bacille Calmette-Guérin by Cathelicidin in Bladder Cancer Cells PubMed: 25681250
Sarcoidosis Vasc Diffuse Lung Dis Cathelicidin as a link between sarcoidosis and tuberculosis PubMed: 26422567
Allergy and Asthma Proceedings The association of vitamin D, cathelicidin, and vitamin D binding protein with acute asthma attacks in children PubMed: 26108071
J Clin Microbiol. Swiftly Decreasing Cerebrospinal Fluid Cathelicidin Concentration Predicts Improved Outcome in Childhood Bacterial Meningitis. Pubmed:27008883
experimental dermatology Light-emitting diodes downregulate cathelicidin, kallikrein and toll-like receptor 2 expressions in keratinocytes and rosacea-like mouse skin. pubmed:27315464
PLOS ONE Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated withActivation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension. pubmed:27391973
Front Pharmacol.  Ginsenoside Rg5 Inhibits Succinate-Associated Lipolysis in Adipose Tissue and Prevents Muscle Insulin Resistance. pubmed:28261091
Allergologia et immunopathologia The relation of innate and adaptive immunity with viral-induced acute asthma attacks: Focusing on IP-10 and cathelicidin pubmed:27955890
Chinese Journal of Osteoporosis and Bone Mineral Research Effects of cold exposure on the bone marrow adipose tissue DOI: 10.3969/j.issn.1674-2591.2017.05.008
Scientific Reports Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping technique Pubmed:29374283
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