ELISA Kit DIY Materials for Apolipoprotein A4 (APOA4)

ApoA-IV; ApoAIV; Apo-A4

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Apolipoprotein A4 (APOA4).No significant cross-reactivity or interference between Apolipoprotein A4 (APOA4) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Biochimica et Biophysica Acta Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties PubMed: 23328279
Exp Ther Med. Differential plasma proteome analysis in patients with high?altitude pulmonary edema at the acute and recovery phases Pubmed:24940404
EuPA Open Proteomics Human maternal plasma proteomic changes with parturition Sciencedirect:S2212968514000506
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