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ELISA Kit DIY Materials for Calreticulin (CALR)

CRT; RO; SSA; CC1qR; ERp60; HACBP; grp60; CRTC; CRP55; Calregulin; Sicca Syndrome Antigen A; Autoantigen Ro; Endoplasmic reticulum resident protein 60

  • Product No.KSB486Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.067ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1231  US$ 2052  US$ 3694  US$ 7182  US$ 12312 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Calreticulin (CRT). No significant cross-reactivity or interference between Calreticulin (CRT) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of Investigative Dermatology Oxidative Stress-Induced Calreticulin Expression and Translocation: New Insights into the Destruction of Melanocytes Pubmed: 23771121
International Journal of Molecular Sciences The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC Mdpi: Source
The Journal of Maternal-Fetal & Neonatal Medicine Amniotic fluid calreticulin in pregnancies complicated by the preterm prelabor rupture of membranes Pubmed:26953684
journal of maternal-fetal & neonatal medicine Cervical fluid calreticulin and cathepsin-G in pregnancies complicated by preterm prelabor rupture of membranes. pubmed:28152632
Talanta Investigation of the interaction between calreticulin and immunoglobulin G by capillary electrophoresis and computer modeling Doi: 10.1016/j.talanta.2018.11.098
Sci Adv Thermostable ionizable lipid-like nanoparticle (iLAND) for RNAi treatment of hyperlipidemia Pubmed:35171673
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB486Mu02 Recombinant Calreticulin (CALR) Positive Control; Immunogen; SDS-PAGE; WB.
RPB486Mu01 Recombinant Calreticulin (CALR) Positive Control; Immunogen; SDS-PAGE; WB.
PAB486Mu01 Polyclonal Antibody to Calreticulin (CALR) WB; IHC; ICC; IP.
PAB486Mu02 Polyclonal Antibody to Calreticulin (CALR) WB; IHC; ICC; IP.
SEB486Mu ELISA Kit for Calreticulin (CALR) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB486Mu Multiplex Assay Kit for Calreticulin (CALR) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB486Mu01 ELISA Kit DIY Materials for Calreticulin (CALR) Main materials for "Do It (ELISA Kit) Yourself".