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ELISA Kit DIY Materials for Alkaline Phosphatase, Intestinal (ALPI)

CIP; IAP; Calf Intestinal Alkaline Phosphatase

  • Product No.KSB085Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.63ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1197  US$ 1995  US$ 3591  US$ 6983  US$ 11970 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of  Alkaline Phosphatase, Intestinal (ALPI). No significant cross-reactivity or interference between  Alkaline Phosphatase, Intestinal (ALPI) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
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Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB085Hu01 Recombinant Alkaline Phosphatase, Intestinal (ALPI) Positive Control; Immunogen; SDS-PAGE; WB.
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PAB085Hu01 Polyclonal Antibody to Alkaline Phosphatase, Intestinal (ALPI) WB; IHC; ICC; IP.
MAB085Hu22 Monoclonal Antibody to Alkaline Phosphatase, Intestinal (ALPI) WB; IHC; ICC; IP.
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KSB085Hu01 ELISA Kit DIY Materials for Alkaline Phosphatase, Intestinal (ALPI) Main materials for "Do It(ELISA Kit) Yourself"