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ELISA Kit DIY Materials for S100 Calcium Binding Protein A12 (S100A12)

CGRP; P6; CAAF1; CAGC; ENRAGE; MRP6; Calgranulin C; Calcium-binding protein in amniotic fluid 1; Extracellular newly identified RAGE-binding; Migration inhibitory factor-related 6

  • Product No.KSB080Rb01
  • Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.067ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1300  US$ 2166  US$ 3899  US$ 7581  US$ 12996 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A12 (S100A12). No significant cross-reactivity or interference between S100 Calcium Binding Protein A12 (S100A12) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Inflammation Research Correlation of human S100A12 (EN-RAGE) and high-sensitivity C-reactive protein as gingival crevicular fluid and serum markers of inflammation in chronic periodontitis and type 2 diabetes Pubmed: 24378957
Dig Dis Sci The Circulating Level of Soluble Receptor for Advanced Glycation End Products Displays Different Patterns in Ulcerative Colitis and Crohn’s Disease: A Cross-Sectional Study PubMed: 25757448
The Journal of Clinical Endocrinology & Metabolism Low Endogenous Secretory Receptor for Advanced Glycation End-Products Levels Are Associated With Inflammation and Carotid Atherosclerosis in Prediabetes Pubmed:26885882
Nutrition, Metabolism, and Cardiovascular Diseases High intake of dietary advanced glycation end-products is associated with increased arterial stiffness and inflammation in subjects with type 2 diabetes. pubmed:28958695
The Journal of Clinical Endocrinology & Metabolism HbA1c Identifies Subjects With Prediabetes and Subclinical Left Ventricular Diastolic Dysfunction. pubmed:28973588
Journal of Immunology Research A Granulocyte-Specific Protein S100A12 as a Potential Prognostic Factor Affecting Aggressiveness of Therapy in Patients with Juvenile Idiopathic Arthritis Doi: 10.1155/2018/5349837
Al-Azhar Assiut Medical Journal The role of serum S100A12 (calgranulin C) as a diagnostic marker in Egyptian patients with irritable bowel syndrome and ulcerative colitis Doi: 10.4103/AZMJ.AZMJ_52_18
Acta Diabetologica Analysis of S100A12 plasma levels in hyperlipidemic subjects with or without familial hypercholesterolemia Pubmed: 30963307
Cells 1 h Postload Glycemia Is Associated with Low Endogenous Secretory Receptor for Advanced Glycation End Product Levels and Early Markers of Cardiovascular …
Biomolecules . Multimarker Approach to Identify Patients with Coronary Artery Disease at High Risk for Subsequent Cardiac Adverse Events: The Multi-Biomarker Study Pubmed: 32549327
Mediators of Inflammation Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris
Animals (Basel) The Serum and Saliva Proteome of Dogs with Diabetes Mellitus 33271797
Catalog No. Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species Applications (RESEARCH USE ONLY!)
RPB080Rb01 Recombinant S100 Calcium Binding Protein A12 (S100A12) Positive Control; Immunogen; SDS-PAGE; WB.
PAB080Rb51 Polyclonal Antibody to S100 Calcium Binding Protein A12 (S100A12) WB; IHC; ICC; IP.
PAB080Rb01 Polyclonal Antibody to S100 Calcium Binding Protein A12 (S100A12) WB; IHC; ICC; IP.
SEB080Rb ELISA Kit for S100 Calcium Binding Protein A12 (S100A12) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB080Rb Multiplex Assay Kit for S100 Calcium Binding Protein A12 (S100A12) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB080Rb01 ELISA Kit DIY Materials for S100 Calcium Binding Protein A12 (S100A12) Main materials for "Do It(ELISA Kit) Yourself"