ELISA Kit DIY Materials for Tryptase (TPS)

TPSAB1; Tryptase Alpha/Beta 1; Tryptase Alpha II; Tryptase Beta I; Tryptase-I; Tryptase-II; Tryptase-III

  • Product No.KSB070Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range15.6-1,000pg/mL
  • Applicable Sensitivity5.5pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • DownloadInstruction Manual
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1197  US$ 1995  US$ 3591  US$ 6983  US$ 11970 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Tryptase (TPS)). No significant cross-reactivity or interference between Tryptase (TPS) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of Allergy and Clinical Immunology Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps PubMed: 22534535
J Pediatr. Improved liver function and relieved pruritus after 4-phenylbutyrate therapy in a patient with progressive familial intrahepatic cholestasis type 2 Pubmed:24530123
Mediators of Inflammation Mediators of Mast Cells in Bullous Pemphigoid and Dermatitis Herpetiformis Pubmed:25400334
PLOS ONE A Novel Model of IgE-Mediated Passive Pulmonary Anaphylaxis in Rats Pubmed:25541997
Neurogastroenterology & Motility Peritoneal mast cell degranulation and gastrointestinal recovery in patients undergoing colorectal surgery Pubmed:25677271
Mediators Inflamm Elevated Serum Tryptase and Endothelin in Patients with ST Segment Elevation Myocardial Infarction: Preliminary Report PubMed: 26089601
Int Immunopharmacol Tryptase and protease-activated receptor-2 stimulate scratching behavior in a murine model of ovalbumin-induced atopic-like dermatitis PubMed: 26049029
Mediators of Inflammation Altered expression of IFN-λ2 in allergic airway disorders and identification of its cell origins Journals: Mi
Mediators of Inflammation Altered Expression of IFN-2 in Allergic Airway Disorders and Identification of Its Cell Origins Pubmed:27057098
Analytica Chimica Acta An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis
Journal of Clinical Investigation Dengue virus-elicited tryptase induces endothelial permeability and shock Pubmed: 31265436
Frontiers in Physiology Mast Cells in Peritoneal Fluid From Women With Endometriosis and Their Possible Role in Modulating Sperm Function Pubmed: 31998139
Biomarkers of Allergic Asthma and their Association with Serum Parameters
Annals of Tropical Medicine & Public Health Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity
Front Immunol Serum CD203c+ Extracellular Vesicle Serves as a Novel Diagnostic and Prognostic Biomarker for Succinylated Gelatin Induced Perioperative Hypersensitive … 34650557
Microbiol Spectr Mast Cell-Derived Proteases Induce Endothelial Permeability and Vascular Damage in Severe Fever with Thrombocytopenia Syndrome Pubmed:35612327
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KSB070Hu01 ELISA Kit DIY Materials for Tryptase (TPS) Main materials for "Do It (ELISA Kit) Yourself".