ELISA Kit DIY Materials for Procollagen I N-Terminal Propeptide (PINP)

P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Procollagen I N-Terminal Propeptide (PINP). No significant cross-reactivity or interference between Procollagen I N-Terminal Propeptide (PINP) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Trials Is there benefit in optimising heart failure treatment in over-80 year-old patients? (HF-80 study): study protocol for a randomized controlled trial. Trials: 1745-6215
Clinical Biochemistry Measurement of aminoterminal propeptide of type I procollagen (PINP) in serum ScienceDirect: S0009912012001543
Therapeutic Apheresis and Dialysis Low molecular weight iron dextran increases fibroblast growth factor-23 concentration, together with parathyroid hormone decrease in hemodialyzed patients PubMed: 22458393
International Journal of Clinical & Experimental Pathology IL4 and IL-17A provide a Th2/Th17-polarized inflammatory milieu in favor of TGF-β1 to induce bronchial epithelial-mesenchymal transition (EMT) PubMed: PMC3726963
Osteoporosis International PINP as a biological response marker during teriparatide treatment for osteoporosis Pubmed: 24599274
Clinical Cancer Research Zoledronic acid has differential anti-tumour activity in the pre-and post-menopausal bone microenvironment in vivo Aacrjournals: Source
Endocrinology Oxytocin Reverses Ovariectomy-Induced Osteopenia and Body Fat Gain Endocrine: Source
general endocrinology Oxytocin reverse ovariectomy-induced Osteoper and body fat gain Pubmed:24506069
J Neurol Sci. The effect of topiramate and lamotrigine on rat bone mass, structure and metabolism. Pubmed:24629477
Bone. Neurofibromin inactivation impairs osteocyte development in Nf1Prx1 and Nf1Col1 mouse models Pubmed:24947449
Journal of Materials Science: Materials in Medicine Phosphatidylethanolamine biomimetic coating increases mesenchymal stem cell osteoblastogenesis Pubmed:24980874
Springerplus. Effects of de-escalated bisphosphonate therapy on bone turnover biomarkers in breast cancer patients with bone metastases Pubmed:25332877
Pharmacology Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy PubMed: 25871861
frontiers in endocrinology Oxytocin reverses osteoporosis in a sex-dependent manner PubMed: 26042090
MERIT RESEARCH JOURNALS Changes in Bone Metabolism Months after Laparoscopic Morbidly Obese Pre-menopausal Women Mms: Content
Heart Rhythm.  Prognostic value of collagen turnover biomarkers in cardiac resynchronization therapy: A subanalysis of the TRUST CRT randomized trial population Pubmed:26776557
BMJ Open Sport Exerc Med Procollagen markers in microdialysate can predict patient outcome after Achilles tendon rupture content:2
Bibliothekssystem Universität Hamburg Bilaterale Kommunikation zwischen Osteoblasten und Osteoklasten volltexte:2016
Inflammation Research Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy pubmed:27516211
Endocrine Bone turnover response is linked to both acute and established metabolic changes in ultra-marathon runners pubmed:27422791
Heart & Vessels Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR pubmed:28004175
journal of science and medicine in sport The effect of calcium and vitamin D supplementation on bone health of male Jockeys. pubmed:27568072
Combined aerobic and resistance training improves bone health of female cancer survivors S2352187216300389
Cellular Physiology and Biochemistry Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy pubmed:27889753
Epilepsy Research The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar rats pubmed:27838501
Sci Rep.  Sprint Interval Training Induces A Sexual Dimorphism but does not Improve Peak Bone Mass in Young and Healthy Mice. pubmed:28303909
Metabolism.  Low periostin levels in adult patients with Langerhans cell histiocytosis are independently associated with the disease activity. pubmed:28521873
Heart and vessels Erratum to: Effects on bone metabolism markers and arterial stiffness by switching to rivaroxaban from warfarin in patients with atrial fibrillation pubmed:28314975
SCIENCE & TECHNOLOGY A Study Protocol: Spinal Morphology, Physical Performance, Quality of Life and Biochemical Markers in Adults at Risk of Osteoporotic Fractures 59f7c85aaca272607e2d8d7a
Knee Surg Sports Traumatol Arthrosc Achilles tendon rupture healing is enhanced by intermittent pneumatic compression upregulating collagen type I synthesis pubmed:28668970
Method for the treatment of cardiovascular fibrosis patents:US9594083
Clinical Cases in Mineral and Bone Metabolism Periostin and sclerostin levels in juvenile Paget's disease pubmed:29263750
Journal of Clinical Research in Pediatric Endocrinology Congenital Hypothyroidism and Bone Remodeling Cycle pubmed:27840329
Biomarkers Medicial Relationship of biomarkers of extracellular matrix with myocardial function in Type 2 diabetes mellitus pubmed:28685602
Heart and Vessels Left ventricular reverse remodeling is not related to biopsy‑detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR 10.1007/s00380-016-0930-y
BioMed Research International Optimization of the Time Window of Interest in Ovariectomized Imprinting Control Region Mice for Antiosteoporosis Research pubmed:29119115
Cytokine 12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both with and without cardiac fibrosis. pubmed:28460256
Advanced Healthcare Materials Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events. pubmed:29120858
Bone. The key role of proinflammatory cytokines, matrix proteins, RANKL/OPG and Wnt/β-catenin in bone healing of hip arthroplasty patients pubmed:29129760
Cell Death And Differentiation TSC1 regulates osteoclast podosome organization and bone resorption through mTORC1 and Rac1/Cdc42 Pubmed:29358671
Nutrients Specific Collagen Peptides Improve Bone Mineral Density and Bone Markers in Postmenopausal Women—A Randomized Controlled Study Pubmed:29337906
Osteoporosis International Two-year cortical and trabecular bone loss in CKD-5D: biochemical and clinical predictors Pubmed:28993865
Journal of Nutrition Cholecalciferol Supplementation Promotes Bone Turnover in Chinese Adults with Vitamin D Deficiency Pubmed:29897564
European Journal of Pharmacology The effect of levetiracetam on rat bone mineral density, bone structure and biochemical markers of bone metabolism Pubmed:29428468
Journal of Clinical Densitometry Geometric and mechanical bone response to a multidisciplinary weight loss intervention in adolescents with obesity: The ADIBOX study Pubmed:30076009
Hernia Circulating matrix metalloproteinases and procollagen propeptides in inguinal hernia Pubmed:29484522
Pharmacology Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats Pubmed:29898457
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
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