ELISA Kit DIY Materials for Nitric Oxide Synthase 2, Inducible (NOS2)

NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Nitric Oxide Synthase 2, Inducible (NOS2).No significant cross-reactivity or interference between Nitric Oxide Synthase 2, Inducible (NOS2) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease High-molecular weight hyaluronan reduced renal PKC activation in genetically diabetic mice PubMed: 20713153
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease Hyaluronan reduces inflammation in experimental arthritis by modulating TLR-2 and TLR-4 cartilage expression ScienceDirect: S0925443911001323
FEBS Journal Adenosine A2a Receptor Activation and Hyaluronan Fragment Inhibition Reduce Inflammation in Mouse Articular Chondrocytes Stimulated with Il-1beta Wiley: source
Gene Hyaluronan in part mediates IL-1beta-induced inflammation in mouse chondrocytes by up-regulating CD44 receptors ScienceDirect: S0378111911007347
PLoS One A Novel Mechanism of Formaldehyde Neurotoxicity: Inhibition of Hydrogen Sulfide Generation by Promoting Overproduction of Nitric Oxide PubMed: 23359814
PLoS ONE Regulation of Microglia Activity by Glaucocalyxin-A: Attenuation of Lipopolysaccharide-Stimulated Neuroinflammation through NF-κB and p38 MAPK Signaling Pathways PlosOne: Source
Journal of Cellular Biochemistry The inhibition of hyaluronan degradation reduced pro-inflammatory cytokines in mouse synovial fibroblasts subjected to collagen-induced arthritis. PubMed: 22234777
International Immunopharmacology Amentoflavone inhibits iNOS, COX-2 expression and modulates cytokine profile, NF-κB signal transduction pathways in rats with ulcerative colitis Pubmed: 24126114
Integrative Cancer Therapies Decalepis hamiltonii inhibits tumor progression and metastasis by regulating the inflammatory mediators and nuclear factor κB subunits Pubmed: 24013642
Food and Chemical Toxicology Inhibition of UVB-induced skin phototoxicity by a grape seed extract as modulator of nitrosative stress, ERK/NF-kB signaling pathway and apoptosis, in SKH-1 mice Pubmed: 23567245
Experimental Gerontology Age-related changes in ADMA–DDAH–NO pathway in rat liver subjected to partial ischemia followed by global reperfusion ScienceDirect: S0531556513003331
International Immunopharmacology Protective effect of marine mangrove Rhizophora apiculata on acetic acid induced experimental colitis by regulating anti-oxidant enzymes, inflammatory mediators and nuclear factor-kappa B subunits ScienceDirect: S1567576913004153
INTERNATIONAL JOURNAL OF MEDICINE AND MEDICAL RESEARCH L-ARGININE, BUT NOT L-NAME PROTECTS AGAINST LIVER INJURY INDUCED BY EXPERIMENTAL ISCHEMIA-REPERFUSION Tdmu:Source
Molecular Vision Effects of topically applied tocotrienol on cataractogenesis and lens redox status in galactosemic rats Pubmed:24940038
Journal of Environmental Pathology, Toxicology and Oncology Protective Effect of?Acacia ferruginea?against Ulcerative Colitis via Modulating Inflammatory Mediators, Cytokine Profile and NF-κB Signal Transduction Pathways Pubmed:24941292
Eur J Cancer Prev. Utility of Apc-mutant rats with a colitis-associated colon carcinogenesis model for chemoprevention studies Pubmed:25222193
Cell and Tissue Research Beta-arrestin 1 is involved in the catabolic response stimulated by hyaluronan degradation in mouse chondrocytes Pubmed:25673209
Ann N Y Acad Sci.? Modulating effect of Biophytum sensitivum extract on rats with acetic acid-induced ulcerative colitis Pubmed:5243883
Molecular Medicine Reports Resveratrol ameliorates oxidative stress and inhibits aquaporin 4 expression following rat cerebral ischemia-reperfusion injury PubMed: 26458999
Chem Biol Interact Apocynin protects against ethanol-induced gastric ulcer in rats by attenuating the upregulation of NADPH oxidases 1 and 4 PubMed: 26522475
Journal of Environmental Pathology, Toxicology and Oncology Protective Effect of PubMed: 26349607
Journal of Immunotoxicology Acacia ferruginea inhibits inflammation by regulating inflammatory iNOS and COX-2 PubMed: 25738525
Cancer Medicine Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host disease Pubmed:27109254
Journal of Natural Medicines Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark of Lycium chinense (Cortex Lycii) against gastric ulcer in mice Pubmed:26972100
Cell Mol Immunol  Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica Pubmed:27157496
Медицина и здравоохранение Роль индуцибельной NO-синтазы в механизмах регуляции функциональной активности кальций-активируемых калиевых каналов коронарных сосудов у крыс, адаптированных к стрессу article:n
Graefe's Archive for Clinical and Experimental Ophthalmology Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndrome pubmed:27538909
Taylor & Francis Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation 09540105.2017.1306494
Molecular Vision Mechanism of the anticataract effect of liposomal MgT in galactose-fed rats. pubmed:27440992
Journal of Natural Medicines Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark ofLycium chinense (Cortex Lycii) against gastric ulcer in mice. pubmed:26972100
Applied Biochemistry and Biotechnology Protective Effect of Zingiber officinale Against Dalton's Lymphoma Ascites Tumour byRegulating Inflammatory Mediator and Cytokines. pubmed:27435276
Cell Mol Neurobiol Posttraumatic Stress Disorder Disturbs Coronary Tone and Its Regulatory Mechanisms pubmed:28676988
Microbial Immunology Immunomodulatory Activities of a Fungal Protein Extracted from Hericium erinaceus through Regulating the Gut Microbiota pubmed:28713364
Biomedicine & Pharmacotherapy Protective effect of Averrhoa bilimbi L. fruit extract on ulcerative colitis in wistar rats via regulation of inflammatory mediators and cytokines pubmed:28531922
The Royal Society of Chemistry Anti-ulcerogenic effect of KFP-H008 against ethanol-induced gastric ulcer via p38 MAPK/NF-κB pathway 10.1039/C7RA08879E
Acta Pharmacologica Sinica Cis-stilbene glucoside in Polygonum multiflorum induces immunological idiosyncratic hepatotoxicity in LPS-treated rats by suppressing PPAR-γ. pubmed:28649126
Cytokine Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effects pubmed:29102164
Molecules Melatonin balance the autophagy and apoptosis by regulating UCP2 in the LPS-induced cardiomyopathy Pubmed:29547569
European Journal of Pharmacology  Naringenin ameliorates learning and memory impairment following systemic lipopolysaccharide challenge in the rat Pubmed:29518393
Regulatory Toxicology and Pharmacology Targeted inhibition of tumor survival, metastasis and angiogenesis by Acacia ferruginea mediated regulation of VEGF, inflammatory mediators, cytokine profile and … Pubmed:29678767
Pharmacological Reports The Association between Inflammatory Markers (iNOS, HO-1, IL-33, MIP-1β) and Depression with and without Posttraumatic Stress Disorder 10.1016:j.pharep.2018.06.001
Clinical And Experimental Pharmacology And Physiology  Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway Pubmed:29266336
Stężenia śródbłonkowej i indukowalnej formy syntazy tlenku azotu w surowicy krwi kobiet ciężarnych palących tytoń–badania wstępne
Journal of Allergy and Clinical Immunology Exosome swarms eliminate airway pathogens and provide passive epithelial immunoprotection through nitric oxide Pubmed: 30442371
Metabolic Brain Disease Trigonelline protects hippocampus against intracerebral Aβ (1–40) as a model of Alzheimer's disease in the rat: insights into underlying mechanisms Pubmed: 30421246
PLoS One Intraphagolysosomal conditions predispose to Staphylococcus epidermidis small colony variants persistence in macrophages Pubmed: 30412620
The Eurasian Journal of Medicine Tadalafil Preserves Penile Nitric Oxide Synthase from Detrimental Effect of Paroxetine in Rats Doi: 10.5152/eurasianjmed.2018.18160
PLoS One Macrophage expression of E3 ubiquitin ligase Grail protects mice from lipopolysaccharide-induced hyperinflammation and organ injury Pubmed: 30571701
International Journal of Environmental Research and Public Health Influence of Active Exposure to Tobacco Smoke on Nitric Oxide Status of Pregnant Women Pubmed: 30513890
Journal of Functional Foods Complex interaction of dietary fat and Alaskan bog blueberry supplementation influences manganese mediated neurotoxicity and behavioral impairments
Particle and Fibre Toxicology Silica nanoparticles trigger the vascular endothelial dysfunction and prethrombotic state via miR-451 directly regulating the IL6R signaling pathway Pubmed: 30975181
ВЛИЯНИЕ ИНДУЦИБЕЛЬНОЙ NO-СИНТАЗЫ НА ТОНУС КОРОНАРНЫХ СОСУДОВ, СОКРАТИТЕЛЬНУЮ ФУНКЦИЮ МИОКАРДА И МЕХАНИЗМЫ …
Journal of Cosmetic Dermatology Whitening effects of cosmetic formulation in the vascular component of skin pigmentation Pubmed: 31074159
regional hemodynamics and microcirculation Benzimidazole Derivative, 2-ETH Prevents Poststressor Disorders of Coronary Vascular Tone and Myocardial Contractility
Life Sci Cilostazol mitigates mesenteric ischemia/reperfusion-induced lung lesion: Contribution of PPAR-¦Ã, NF-¦ÊB, and STAT3 crosstalk 33310046
PLoS One Correlation analysis of the proportion of monocytic myeloid-derived suppressor cells in colorectal cancer patients 33370317
Biotechnol Rep Targeting NF-¦ÊB mediated cell signaling pathway and inflammatory mediators by 1, 2-diazole in A549 cells in vitro 33598414
Basic and Clinical Neuroscience Paeonol Protects Against Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson's Disease 33995926
Fermented Foods and Microbes Related to Health Cordyceps cicadae NTTU 868 Mycelium with The Addition of Bioavailable Forms of Magnesium from Deep Ocean Water Prevents the A¦Â40 and Streptozotocin?¡­
Ya?lanma ve Hipertansiyonda Yeni bir Endoltelyal Disfonksiyon Belirteci: ??z¨¹nebilir Lektin Benzeri Okside D¨¹?¨¹k Dansiteli Lipoprotein-1
Fermentation Cordyceps cicadae NTTU 868 Mycelium with The Addition of Bioavailable Forms of Magnesium from Deep Ocean Water Prevents the A¦Â40 and Streptozotocin-Induced Memory Deficit via Suppressing Alzheimer¡¯s Disease Risk Factors and Increasing Magnesium Uptake of Brain
Drug Chem Toxicol Study of cardioprotective activity of the methanolic extract of the aerial parts of Bauhinia madagascariensis compared to Bauhinia purpurea against adrenaline … 34167392
Frontiers in Cardiovascular Medicine The Effects of Intermittent Hypoxic–Hyperoxic Exposures on Lipid Profile and Inflammation in Patients With Metabolic Syndrome 34513946
Nutrients Erinacine A-Enriched Hericium erinaceus Mycelium Delays Progression of Age-Related Cognitive Decline in Senescence Accelerated Mouse Prone 8 (SAMP8) Mice 34684662
biomolecules Whole-Body Cryotherapy Increases the Activity of Nitric Oxide Synthase in Older Men 34356664
Hum Exp Toxicol Carvacrol hinders the progression of hepatic fibrosis via targeting autotaxin and thioredoxin in thioacetamide-induced liver fibrosis in rat 34155936
J Ethnopharmacol Post-stroke treatment of storax improves long-term outcomes of stroke in rats 34343648
Phytomedicine A multidimensional strategy for uncovering comprehensive quality markers of Schisandra chinensis (Turcz.) Baill based on pharmacodynamics and chemical …
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA837Mu03 Recombinant Nitric Oxide Synthase 2, Inducible (NOS2) Positive Control; Immunogen; SDS-PAGE; WB.
APA837Mu01 Active Nitric Oxide Synthase 2, Inducible (NOS2) Cell culture; Activity Assays.
RPA837Mu01 Recombinant Nitric Oxide Synthase 2, Inducible (NOS2) Positive Control; Immunogen; SDS-PAGE; WB.
RPA837Mu02 Recombinant Nitric Oxide Synthase 2, Inducible (NOS2) Positive Control; Immunogen; SDS-PAGE; WB.
PAA837Mu01 Polyclonal Antibody to Nitric Oxide Synthase 2, Inducible (NOS2) WB; IHC; ICC; IP.
PAA837Mu02 Polyclonal Antibody to Nitric Oxide Synthase 2, Inducible (NOS2) WB; IHC; ICC; IP.
PAA837Mu03 Polyclonal Antibody to Nitric Oxide Synthase 2, Inducible (NOS2) WB; IHC; ICC; IP.
LAA837Mu71 Biotin-Linked Polyclonal Antibody to Nitric Oxide Synthase 2, Inducible (NOS2) WB; IHC; ICC.
SEA837Mu ELISA Kit for Nitric Oxide Synthase 2, Inducible (NOS2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA837Mu Multiplex Assay Kit for Nitric Oxide Synthase 2, Inducible (NOS2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA837Mu01 ELISA Kit DIY Materials for Nitric Oxide Synthase 2, Inducible (NOS2) Main materials for "Do It (ELISA Kit) Yourself".