ELISA Kit DIY Materials for Heat Shock Protein 60 (Hsp60)

HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1, Chaperonin; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein

  • Product No.KSA822Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.64ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1134  US$ 1890  US$ 3402  US$ 6615  US$ 11340 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of  Heat Shock 60kD Protein 1, Chaperonin (HSPD1). No significant cross-reactivity or interference between  Heat Shock 60kD Protein 1, Chaperonin (HSPD1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support Pack

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Sci Rep Hsp60 response in experimental and human temporal lobe epilepsy PubMed: 25801186
Cellular and Molecular Gastroenterology and Hepatology Senescent hepatocytes in decompensated liver show reduced UPR and its key player, CLPP, attenuates senescence in vitro Pubmed: 30878663
Breast Cancer Res Treat Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancer Pubmed: 32601970
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA822Hu02 Recombinant Heat Shock Protein 60 (Hsp60) Positive Control; Immunogen; SDS-PAGE; WB.
RPA822Hu01 Recombinant Heat Shock Protein 60 (Hsp60) Positive Control; Immunogen; SDS-PAGE; WB.
PAA822Hu01 Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) WB,IHC,ICC/IF
PAA822Hu02 Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) WB; IHC; ICC; IP.
FAA822Hu01 Anti-Heat Shock Protein 60 (Hsp60) Polyclonal Antibody Flow cytometry.
LAA822Hu81 FITC-Linked Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) WB; IHC; ICC; IF.
LAA822Hu71 Biotin-Linked Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) WB; IHC; ICC.
FAA822Hu41 PE-Linked Anti-Heat Shock Protein 60 (Hsp60) Polyclonal Antibody Flow cytometry.
MAA822Hu22 Monoclonal Antibody to Heat Shock Protein 60 (Hsp60) WB; IHC; ICC; IP.
MAA822Hu21 Monoclonal Antibody to Heat Shock Protein 60 (Hsp60) WB; IHC; ICC; IP.
MAA822Hu23 Monoclonal Antibody to Heat Shock Protein 60 (Hsp60) WB; IHC; ICC; IP.
MAA822Hu24 Monoclonal Antibody to Heat Shock Protein 60 (Hsp60) WB; IHC; ICC; IP.
MAA822Hu25 Monoclonal Antibody to Heat Shock Protein 60 (Hsp60) WB; IHC; ICC; IP.
FAA822Hu02 Anti-Heat Shock Protein 60 (Hsp60) Monoclonal Antibody Flow cytometry.
SEA822Hu ELISA Kit for Heat Shock Protein 60 (Hsp60) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA822Hu High Sensitive ELISA Kit for Heat Shock Protein 60 (Hsp60) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA822Hu Multiplex Assay Kit for Heat Shock Protein 60 (Hsp60) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA822Hu01 ELISA Kit DIY Materials for Heat Shock Protein 60 (Hsp60) Main materials for "Do It(ELISA Kit) Yourself"