ELISA Kit DIY Materials for Visfatin (VF)
Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase
- Product No.KSA638Hu11
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range1.23-100ng/mL
- Applicable Sensitivity
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- DownloadInstruction Manual
- UOM 96T*596T*10 96T*20 96T*50 96T*100
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The Abs in the kit have high sensitivity and excellent specificity for detection of Visfatin (VF). No significant cross-reactivity or interference between Visfatin (VF) and analogues was observed.
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
|Brazilian Journal of Biomoricity||EFFECT OF 8 WEEKS ENDURANCE TRAINING ON PLASMA VISFATIN IN MIDDLE-AGED MEN Brjb: source|
|Diabetes Research and Clinical Practice||The relationship between visfatin and HOMA-IR in hypertensive patients, and the effect of antihypertensive drugs on visfatin and HOMA-IR in hypertensive patients with insulin resistance ScienceDirect: S0168822711002956|
|J Physiol Pharmacol.||Early-effect of bariatric surgery (Scopinaro method) on intestinal hormones and adipokines in insulin resistant Wistar rat. Pubmed: 24304571|
|Endocr J.||CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes Pubmed:25168658|
|Exp Ther Med.||Plasma visfatin levels and mRNA expression of visfatin in peripheral blood mononuclear cells and peripheral blood monocyte-derived macrophages from normal weight females with polycystic ovary syndrome Pubmed:Pmc3991522|
|Eur J Cancer Prev.||Visfatin derived from ascites promotes ovarian cancer cell migration through Rho/ROCK signaling-mediated actin polymerization. Pubmed:25055182|
|Genome||Visfatin −948G/T and resistin −420C/Gpolymorphisms in Egyptian type 2 diabetic patients with and without cardiovascular diseases Nrcresearchpress:Source|
|PLOS ONE||Antidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting Drug PubMed: 26262991|
|Alcoholism and Drug Addiction||Changes in concentration of visfatin during four weeks of inpatient treatment of alcohol dependent males Science: Article|
|Cytokine||Variation in blood levels of hormones in obese patients following weight reduction induced by endoscopic and surgical bariatric therapies PubMed: 26539806|
|Genome||Genetic Variations in Visfatin and Resistin Genes in Egyptian Type 2 Diabetic Patients With and Without Complications PubMed: 25120107|
|Acta Histochemica||Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines science:S0065128116301234|
|Endocrine, Metabolic & Immune Disorders-Drug Targets||Changes in Serum Nampt Levels and Its Significances in Diabetic Nephropathy Patients-The Potential Role of Nampt in T2DM with Diabetic Nephropathy. pubmed:28699485|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA638Hu01||Recombinant Visfatin (VF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|CPA638Hu21||OVA Conjugated Visfatin (VF)||Immunogen; SDS-PAGE; WB.|
|PAA638Hu01||Polyclonal Antibody to Visfatin (VF)||WB; IHC; ICC; IP.|
|PAA638Hu08||Polyclonal Antibody to Visfatin (VF)||WB; IHC; ICC; IP.|
|MAA638Hu22||Monoclonal Antibody to Visfatin (VF)||WB; IHC; ICC; IP.|
|SEA638Hu||ELISA Kit for Visfatin (VF)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|CEA638Hu||ELISA Kit for Visfatin (VF)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSA638Hu11||ELISA Kit DIY Materials for Visfatin (VF)||Main materials for "Do It (ELISA Kit) Yourself".|