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ELISA Kit DIY Materials for Dihydrotestosterone (DHT)

5α-DHT; 5α-Dihydrotestosterone; Androstanolone; Stanolone

  • Product No.KSA443Ge11
  • Organism SpeciesPan-species (General) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range30.9-2500pg/mL
  • Applicable Sensitivity13.3pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1433  US$ 2388  US$ 4298  US$ 8358  US$ 14328 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Dihydrotestosterone (DHT). No significant cross-reactivity or interference between Dihydrotestosterone (DHT) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Internation Scholarly Research Network Herbicide Metolachlor Causes Changes in Reproductive Endocrinology of Male Wistar Rats Isrn: 130846
Journal of American Science Metabolic Changes and Hormonal Disturbances in Polycystic Ovarian Syndrome Rats and the Amelioration Effects of Metformin and/or Cinnamon Extraction Jofamericanscience: Source
Journal of Ethnopharmacology Treatment of benign prostatic hyperplasia with Croton membranaceus in an experimental animal model Pubmed:25256687
Toxicol Ind Health Oral administration of low-dose bisphenol A promotes proliferation of ventral prostate and upregulates prostaglandin D2 PubMed: 26088557
PPAR Research Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARα pubmed:27413362
Neurochemical Research Dihydrotestosterone Treatment Accelerates Autograft Reversal Sciatic Nerve Regeneration in Rats Pubmed:29340845
Journal of Endocrinology Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome Pubmed: 29535146
Journal of Cellular Physiology Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression Pubmed: 30671954
PHYTOTHERAPY RESEARCH Plant cell culture extract of Cirsium eriophorum with skin pore refiner activity by modulating sebum production and inflammatory response Pubmed: 32816329
Curr Oncol Plasma Aromatase Activity Index, Gonadotropins and Estrone Are Associated with Frailty Syndrome in Post-Menopausal Women with Breast Cancer Pubmed:35323344
J Steroid Biochem Mol Biol Syce1 and Syce3 regulate testosterone and dihydrotestosterone synthesis via steroidogenic pathways in mouse Sertoli and Leydig cells Pubmed:35697131
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CPA443Ge11 BSA Conjugated Dihydrotestosterone (DHT) Immunogen; SDS-PAGE; WB.
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IEA443Ge Instant ELISA Kit for Dihydrotestosterone (DHT) Enzyme-linked immunosorbent assay for Antigen Detection.
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LMA443Ge Multiplex Assay Kit for Dihydrotestosterone (DHT) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA443Ge11 ELISA Kit DIY Materials for Dihydrotestosterone (DHT) Main materials for "Do It (ELISA Kit) Yourself".