ELISA Kit DIY Materials for Interleukin 1 Receptor Antagonist (IL1RA)

IL1RN; IL1-RA; ICIL1RA; IL1ra3; IL1F3; IRAP;IL-1RA; Interleukin-1 Family Member 3; IL1 inhibitor; Anakinra

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 1 Receptor Antagonist (IL1RA). No significant cross-reactivity or interference between Interleukin 1 Receptor Antagonist (IL1RA) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
The Veterinary Journal Mucosal imbalance of interleukin-1β and interleukin-1 receptor antagonist in canine inflammatory bowel disease ScienceDirect: S1090023312000962
Daily quercetin supplementation over a period of two weeks results in a moderate accumulation of total plasma flavonols in horses Researchgate:Source
Journal of Equine Veterinary Science Synovial Fluid and Serum Concentrations of Interleukin-1 Receptor Antagonist and Interleukin-1 Science: Article
Journal of Equine Veterinary Science A Two-Week Quercetin Supplementation in Horses Results in Moderate Accumulation of Plasma Flavonol Concentrations Science: Article
Scientific publications in peer reviewed journals Evidence-based review of efficacy and adverse effects of joint medication and evaluation of synovial fluid and serum markers for osteoarthritis in the horse receive:101658
Multiple Sclerosis and Related Disorders Interleukin 1 receptor antagonist and 2′-5′-oligoadenylate synthetase-like molecules as novel biomarkers for multiple sclerosis patients in Bahrain pubmed:29141788
Advances in Medical Sciences A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmatics pubmed:28822267
Archives of Medical Research Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young … Pubmed:29379533
Journal of Veterinary Internal Medicine Effect of interleukin‐1β on occludin mRNA expression in the duodenal and colonic mucosa of dogs with inflammatory bowel disease Pubmed:29572935
Frontiers in Pharmacology M10, a Myricetin-3-ObD-Lactose Sodium Salt, Prevents Ulcerative Colitis Through Inhibiting Necroptosis in Mice Pubmed: 33041798
Catalog No. Related products for research use of Sus scrofa; Porcine (Pig) Organism species Applications (RESEARCH USE ONLY!)
RPA223Po01 Recombinant Interleukin 1 Receptor Antagonist (IL1RA) Positive Control; Immunogen; SDS-PAGE; WB.
PAA223Po01 Polyclonal Antibody to Interleukin 1 Receptor Antagonist (IL1RA) WB; IHC; ICC; IP.
LAA223Po81 FITC-Linked Polyclonal Antibody to Interleukin 1 Receptor Antagonist (IL1RA) WB; IHC; ICC; IF.
LAA223Po71 Biotin-Linked Polyclonal Antibody to Interleukin 1 Receptor Antagonist (IL1RA) WB; IHC; ICC.
MAA223Po22 Monoclonal Antibody to Interleukin 1 Receptor Antagonist (IL1RA) WB; IHC; ICC; IP.
KSA223Po01 ELISA Kit DIY Materials for Interleukin 1 Receptor Antagonist (IL1RA) Main materials for "Do It(ELISA Kit) Yourself"